A0A178U700 · A0A178U700_ARATH

Function

function

Dol-P-Glc:Glc2Man9GlcNAc2-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc2Man9GlcNAc2-PP-Dol to produce Glc3Man9GlcNAc2-PP-Dol.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Protein modification; protein glycosylation.

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functiondolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity
Biological Processdolichol-linked oligosaccharide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
  • EC number

Gene names

    • ORF names
      AN1_LOCUS21031
      , C24_LOCUS20929
    • Ordered locus names
      AXX17_At5g01540

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Landsberg erecta
    • cv. An-1
    • cv. C24
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    A0A178U700
  • Secondary accessions
    • A0A5S9Y104

Proteomes

Subcellular Location

Endoplasmic reticulum membrane
; Multi-pass membrane protein
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane99-117Helical
Transmembrane137-160Helical
Transmembrane279-299Helical
Transmembrane319-336Helical
Transmembrane356-380Helical
Transmembrane400-422Helical
Transmembrane429-452Helical
Transmembrane472-493Helical

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-20
ChainPRO_503325579421-509Dol-P-Glc:Glc2Man9GlcNAc2-PP-Dol alpha-1,2-glucosyltransferase

Expression

Gene expression databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region210-254Disordered

Sequence similarities

Belongs to the ALG10 glucosyltransferase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    509
  • Mass (Da)
    58,457
  • Last updated
    2016-09-07 v1
  • Checksum
    CFAB88BD3A7408B3
MGKLAVAAITSLWVIPMSIIVNHIVPEPYMDEIFHVPQAQQYCNGNFRSWDPMITTPPGLYYLSLAHVASLFPGMLLMENTSQSFSEACSTSVLRSTNAVSAVLCGVLVYEIIRFLGPNLSDRKATFMALVMSLYPLHWFFTFLYYTDVASLTAVLAMYLTCLKRRYVLSALFGTLAVFIRQTNVVWMLFVACSGILDFTLDSSKQKGKQEVNQELHQSSNKKGATLRSNLRKRKSDISSDTSDPFNHGQTVPSTEDTSDLVYDIYTVISTSWNLKWRILIKFSPFIFVVVAFGIFILWNGGIVLGAKEAHVVSLHFAQIMYFSLVSALFTAPLHFSVNQLRHQFHQLHRNWSLSLILTLVALVAGFVSVHFFSLAHPYLLADNRHYPFYLWRKIINAHWLMKYMLVPVYVYSWFSILTLLAKTRRQTWILVYFLATCGVLVPTPLIEFRYYTIPFYLFMLHSCVRSSSFATWLLIGTIFVSINVFTMAMFLFRPFKWSHEDGVQRFIW

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CACSHJ010000096
EMBL· GenBank· DDBJ
CAA0400194.1
EMBL· GenBank· DDBJ
Genomic DNA
LUHQ01000005
EMBL· GenBank· DDBJ
OAO89676.1
EMBL· GenBank· DDBJ
Genomic DNA
CACRSJ010000110
EMBL· GenBank· DDBJ
VYS65625.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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