A0A177ZXH9 · A0A177ZXH9_9BACI
- ProteinCarbamoyl phosphate synthase small chain
- GenecarA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids366 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.
Catalytic activity
- hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H+
- L-glutamine + H2O = L-glutamate + NH4+
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 45 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 219 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 221 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 247 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 250 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 288 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 290 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 291 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 333 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | carbamoyl-phosphate synthase complex | |
Molecular Function | ATP binding | |
Molecular Function | carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity | |
Molecular Function | glutaminase activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process | |
Biological Process | arginine biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyl phosphate synthase small chain
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Lederbergia
Accessions
- Primary accessionA0A177ZXH9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-131 | Carbamoyl-phosphate synthase small subunit N-terminal | |||
Region | 1-170 | CPSase | |||
Sequence similarities
Belongs to the CarA family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length366
- Mass (Da)40,469
- Last updated2016-09-07 v1
- ChecksumCA702399AAD137C0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LDJR01000037 EMBL· GenBank· DDBJ | OAK72611.1 EMBL· GenBank· DDBJ | Genomic DNA |