A0A177ZP85 · A0A177ZP85_9BACI

Function

function

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site27ATP (UniProtKB | ChEBI)
Binding site27CTP (UniProtKB | ChEBI)
Binding site30ATP (UniProtKB | ChEBI)
Binding site30CTP (UniProtKB | ChEBI)
Binding site40Mg2+ (UniProtKB | ChEBI)
Binding site42Mg2+ (UniProtKB | ChEBI)
Binding site111ATP (UniProtKB | ChEBI)
Binding site111CTP (UniProtKB | ChEBI)
Binding site154ATP (UniProtKB | ChEBI)
Binding site154CTP (UniProtKB | ChEBI)
Binding site157ATP (UniProtKB | ChEBI)
Binding site157CTP (UniProtKB | ChEBI)
Binding site160ATP (UniProtKB | ChEBI)
Binding site160CTP (UniProtKB | ChEBI)
Binding site163ATP (UniProtKB | ChEBI)
Binding site163CTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular Functionmagnesium ion binding
Molecular FunctiontRNA binding
Biological ProcessRNA repair
Biological ProcesstRNA 3'-terminal CCA addition

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CCA-adding enzyme
  • EC number
  • Alternative names
    • CCA tRNA nucleotidyltransferase
    • tRNA CCA-pyrophosphorylase
    • tRNA adenylyl-/cytidylyl- transferase
    • tRNA nucleotidyltransferase
    • tRNA-NT

Gene names

    • Name
      cca
    • ORF names
      ABB05_13520

Organism names

Accessions

  • Primary accession
    A0A177ZP85

Proteomes

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain22-141Poly A polymerase head
Domain169-225tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding
Domain248-391CCA-adding enzyme C-terminal

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    405
  • Mass (Da)
    47,598
  • Last updated
    2016-09-07 v1
  • Checksum
    68914EA5E773EA8A
MDRLFIEAKPVIQQLKNAGYEAYYVGGAVRDYLLKREIHDIDIATSATPLEIKSVFPTTVDVGIEHGTILVIYEKKGYEITTFRAETKYIDFRRPEDVMFIRSLKEDLQRRDFTINAMAMDEEGNIIDPFNGQEDIKKKCIKTVGSPTERFGEDALRLMRAVRFVSQLGFKLDMGTEEALIEQAPLLQYIAIERILMEMEKLLTGKYVKQALKIARKTQLHEYWPAIFKQDHVIDQIVEMPIEELDNRHLWLLALYLCKTEEPQKQFKKWKMPMKEIKKLLRALTFLNWRMNSKWTNVQLYRAGKEIAVMVEKLVQVLQYEKPQSIEAIIDKKFAEFPITDRSHLAITGTDILEWTEQTPGPWVSQLLNQLEEEILNKNLPNEREMIRRWVKGWENQSRDKCSKH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LDJR01000053
EMBL· GenBank· DDBJ
OAK69796.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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