A0A176Q9V4 · A0A176Q9V4_9MICO

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site16[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site45(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site45dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site45isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site78(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site78dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site78isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site100[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site128(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site128dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site128isopentenyl diphosphate (UniProtKB | ChEBI)
Active site130Proton donor
Binding site168(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site198[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site226(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site226dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site226isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site227(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site227dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site227isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site228(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site228dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site228isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site271(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site271dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site271isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      AWH69_14085

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • CD11-4
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Micrococcales > Intrasporangiaceae > Janibacter

Accessions

  • Primary accession
    A0A176Q9V4

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the IspH family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    337
  • Mass (Da)
    36,685
  • Last updated
    2016-09-07 v1
  • Checksum
    E13B7A23704DE543
MSDSPRVLLAAPRGYCAGVDRAVVAVEKALELYGPPVYVRKEIVHNKHVVTTLEKRGAIFVDETGEVPEGATVVFSAHGVAPVVHEEAKALSLKTIDATCPLVTKVHREAVRFAADDFDILLIGHEGHEEVVGTSGEAPEHITLVDGPDHVAEIEVRDPEKVVWLSQTTLSVDETMETVRRLREKFPALQDPPSDDICYATQNRQLAVKQMAAQCDLMIVVGSRNSSNSVRLVEVALEHGTRDGHLVDYAEEIDPAWFEGVRTVGVTSGASVPEILVRDVLALLAEHGYADVEPVVAAEESLLFALPMELRRDLKAAGMDDKMRHDAGSLEDAGQLR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LQZG01000004
EMBL· GenBank· DDBJ
OAB86455.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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