A0A174K775 · A0A174K775_BACUN

  • Protein
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • Gene
    pfkA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Non-allosteric.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site78diphosphate (UniProtKB | ChEBI)
Binding site172Mg2+ (UniProtKB | ChEBI); catalytic
Site173Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site199Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site200-202substrate; ligand shared between dimeric partners; in other chain
Active site202Proton acceptor
Binding site239-240substrate; ligand shared between dimeric partners
Binding site247-249substrate; ligand shared between dimeric partners; in other chain
Binding site308substrate; ligand shared between dimeric partners; in other chain
Binding site421-424substrate; ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processresponse to glucose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • EC number
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-dependent phosphofructokinase
      (PPi-PFK
      )
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      pfkA
    • Synonyms
      pfkA_1
      , pfp
    • ORF names
      B5G17_09260
      , BHV79_14600
      , Bun01g_21510
      , CE91St12_21750
      , DW729_06125
      , DW758_12940
      , DW795_07460
      , DW873_11325
      , DW988_13655
      , DWW14_04250
      , DWW83_18365
      , DWX87_03640
      , DXC07_13165
      , DXD40_00585
      , DXD90_02690
      , ERS417307_01975
      , ERS852462_01701
      , ERS852510_00750
      , ERS852554_02695
      , GAP48_11380
      , GAP55_05770
      , GAQ34_06990
      , GAQ44_17705
      , GAQ56_12710
      , GAQ59_10005
      , GAQ70_07065
      , GAQ72_06130
      , GAQ75_11475
      , HUV07_16290
      , POY80_10085
      , RVH16_16490

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • 2789STDY5608791
    • 2789STDY5834847
    • 2789STDY5834898
    • 2789STDY5834942
    • 45_41
  • Taxonomic lineage
    Bacteria > Bacteroidota > Bacteroidia > Bacteroidales > Bacteroidaceae > Bacteroides

Accessions

  • Primary accession
    A0A174K775

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain70-342Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    548
  • Mass (Da)
    60,119
  • Last updated
    2016-09-07 v1
  • Checksum
    7CFBAB683788C8E7
MTKSALQIARAAYQPKLPKALKGTVKAAEGAATQSVADQEEIKKLFPNTYGMPLIKFETTDEVVNFPAMNVGVILSGGQAPGGHNVISGIFDGIKKLNKDSKLYGFILGPGGLVDHNYMELTADIIDEYRNTGGFDIIGSGRTKLEKEEQFEKGYEILKELGIKALVIIGGDDSNTNACVLAEYYAAKNYGVQVIGCPKTIDGDLKNDMIETSFGFDTACKTYSEVIGNIQRDCNSARKYWHFIKLMGRSASHIALECALQVQPNMCIISEEVEAKDMSLDDIVTSIAKVVAERAAQGNNFGTVLIPEGLVEFIPAMKRLIAELNDFLAANAEEFAQIKKSHQRDYIIRKLSPENAAIYASLPEGVARQLSLDRDPHGNVQVSLIETEKLLSEMVGTKLAQWKEEGKFVGKFAAQHHFFGYEGRCAAPSNFDADYCYSLGYAASALIANGKTGYMSSVRNTTAPAEEWIAGGVPITMMMNMERRHGEMKPVIQKALVKLDGAPFKAFAAQRDRWAMETDYVYPGPIQYFGPTEVCDQATKTLQLEQAK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP019724
EMBL· GenBank· DDBJ
BBK87781.1
EMBL· GenBank· DDBJ
Genomic DNA
CYZF01000005
EMBL· GenBank· DDBJ
CUO59254.1
EMBL· GenBank· DDBJ
Genomic DNA
CZAF01000004
EMBL· GenBank· DDBJ
CUO82059.1
EMBL· GenBank· DDBJ
Genomic DNA
CZAO01000003
EMBL· GenBank· DDBJ
CUP06671.1
EMBL· GenBank· DDBJ
Genomic DNA
CZBF01000004
EMBL· GenBank· DDBJ
CUQ03493.1
EMBL· GenBank· DDBJ
Genomic DNA
BQNL01000001
EMBL· GenBank· DDBJ
GKH13965.1
EMBL· GenBank· DDBJ
Genomic DNA
WCUV01000008
EMBL· GenBank· DDBJ
KAB4090842.1
EMBL· GenBank· DDBJ
Genomic DNA
WCUP01000004
EMBL· GenBank· DDBJ
KAB4110336.1
EMBL· GenBank· DDBJ
Genomic DNA
WCUR01000012
EMBL· GenBank· DDBJ
KAB4118280.1
EMBL· GenBank· DDBJ
Genomic DNA
WCUQ01000006
EMBL· GenBank· DDBJ
KAB4124225.1
EMBL· GenBank· DDBJ
Genomic DNA
WCUG01000007
EMBL· GenBank· DDBJ
KAB4170562.1
EMBL· GenBank· DDBJ
Genomic DNA
WCTY01000037
EMBL· GenBank· DDBJ
KAB4180908.1
EMBL· GenBank· DDBJ
Genomic DNA
WCUA01000005
EMBL· GenBank· DDBJ
KAB4186613.1
EMBL· GenBank· DDBJ
Genomic DNA
WCTR01000004
EMBL· GenBank· DDBJ
KAB4214154.1
EMBL· GenBank· DDBJ
Genomic DNA
WCTJ01000017
EMBL· GenBank· DDBJ
KAB4253071.1
EMBL· GenBank· DDBJ
Genomic DNA
JAQNQY010000009
EMBL· GenBank· DDBJ
MDC1752789.1
EMBL· GenBank· DDBJ
Genomic DNA
JAWDEU010000002
EMBL· GenBank· DDBJ
MDU0246292.1
EMBL· GenBank· DDBJ
Genomic DNA
JABWDF010000149
EMBL· GenBank· DDBJ
NUO13720.1
EMBL· GenBank· DDBJ
Genomic DNA
MNQU01000270
EMBL· GenBank· DDBJ
OKZ31003.1
EMBL· GenBank· DDBJ
Genomic DNA
NFHS01000004
EMBL· GenBank· DDBJ
OUN54708.1
EMBL· GenBank· DDBJ
Genomic DNA
QSOF01000002
EMBL· GenBank· DDBJ
RGI79792.1
EMBL· GenBank· DDBJ
Genomic DNA
QSPV01000001
EMBL· GenBank· DDBJ
RGJ96943.1
EMBL· GenBank· DDBJ
Genomic DNA
QSTL01000012
EMBL· GenBank· DDBJ
RGM54247.1
EMBL· GenBank· DDBJ
Genomic DNA
QRVP01000002
EMBL· GenBank· DDBJ
RGS57127.1
EMBL· GenBank· DDBJ
Genomic DNA
QRXV01000023
EMBL· GenBank· DDBJ
RGU36061.1
EMBL· GenBank· DDBJ
Genomic DNA
QRZC01000003
EMBL· GenBank· DDBJ
RGV45282.1
EMBL· GenBank· DDBJ
Genomic DNA
QSEE01000013
EMBL· GenBank· DDBJ
RGZ47445.1
EMBL· GenBank· DDBJ
Genomic DNA
QSHA01000007
EMBL· GenBank· DDBJ
RHB72920.1
EMBL· GenBank· DDBJ
Genomic DNA
QSJC01000006
EMBL· GenBank· DDBJ
RHD40472.1
EMBL· GenBank· DDBJ
Genomic DNA
QSJZ01000010
EMBL· GenBank· DDBJ
RHE22623.1
EMBL· GenBank· DDBJ
Genomic DNA
QSKL01000003
EMBL· GenBank· DDBJ
RHE60780.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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