A0A174K775 · A0A174K775_BACUN
- ProteinPyrophosphate--fructose 6-phosphate 1-phosphotransferase
- GenepfkA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids548 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.
Catalytic activity
- beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose 1,6-bisphosphate + phosphate + H+
Cofactor
Activity regulation
Non-allosteric.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 78 | diphosphate (UniProtKB | ChEBI) | |||
Binding site | 172 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Site | 173 | Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP | |||
Site | 199 | Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi | |||
Binding site | 200-202 | substrate; ligand shared between dimeric partners; in other chain | |||
Active site | 202 | Proton acceptor | |||
Binding site | 239-240 | substrate; ligand shared between dimeric partners | |||
Binding site | 247-249 | substrate; ligand shared between dimeric partners; in other chain | |||
Binding site | 308 | substrate; ligand shared between dimeric partners; in other chain | |||
Binding site | 421-424 | substrate; ligand shared between dimeric partners; in other chain | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | diphosphate-fructose-6-phosphate 1-phosphotransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process | |
Biological Process | response to glucose |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyrophosphate--fructose 6-phosphate 1-phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacteroidota > Bacteroidia > Bacteroidales > Bacteroidaceae > Bacteroides
Accessions
- Primary accessionA0A174K775
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 70-342 | Phosphofructokinase | |||
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'Long' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length548
- Mass (Da)60,119
- Last updated2016-09-07 v1
- Checksum7CFBAB683788C8E7
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP019724 EMBL· GenBank· DDBJ | BBK87781.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CYZF01000005 EMBL· GenBank· DDBJ | CUO59254.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CZAF01000004 EMBL· GenBank· DDBJ | CUO82059.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CZAO01000003 EMBL· GenBank· DDBJ | CUP06671.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CZBF01000004 EMBL· GenBank· DDBJ | CUQ03493.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BQNL01000001 EMBL· GenBank· DDBJ | GKH13965.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCUV01000008 EMBL· GenBank· DDBJ | KAB4090842.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCUP01000004 EMBL· GenBank· DDBJ | KAB4110336.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCUR01000012 EMBL· GenBank· DDBJ | KAB4118280.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCUQ01000006 EMBL· GenBank· DDBJ | KAB4124225.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCUG01000007 EMBL· GenBank· DDBJ | KAB4170562.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCTY01000037 EMBL· GenBank· DDBJ | KAB4180908.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCUA01000005 EMBL· GenBank· DDBJ | KAB4186613.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCTR01000004 EMBL· GenBank· DDBJ | KAB4214154.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WCTJ01000017 EMBL· GenBank· DDBJ | KAB4253071.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAQNQY010000009 EMBL· GenBank· DDBJ | MDC1752789.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAWDEU010000002 EMBL· GenBank· DDBJ | MDU0246292.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JABWDF010000149 EMBL· GenBank· DDBJ | NUO13720.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MNQU01000270 EMBL· GenBank· DDBJ | OKZ31003.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NFHS01000004 EMBL· GenBank· DDBJ | OUN54708.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSOF01000002 EMBL· GenBank· DDBJ | RGI79792.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSPV01000001 EMBL· GenBank· DDBJ | RGJ96943.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSTL01000012 EMBL· GenBank· DDBJ | RGM54247.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QRVP01000002 EMBL· GenBank· DDBJ | RGS57127.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QRXV01000023 EMBL· GenBank· DDBJ | RGU36061.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QRZC01000003 EMBL· GenBank· DDBJ | RGV45282.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSEE01000013 EMBL· GenBank· DDBJ | RGZ47445.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSHA01000007 EMBL· GenBank· DDBJ | RHB72920.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSJC01000006 EMBL· GenBank· DDBJ | RHD40472.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSJZ01000010 EMBL· GenBank· DDBJ | RHE22623.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QSKL01000003 EMBL· GenBank· DDBJ | RHE60780.1 EMBL· GenBank· DDBJ | Genomic DNA |