A0A172YYU0 · A0A172YYU0_9PSED
- ProteinNADPH-dependent 7-cyano-7-deazaguanine reductase
- GenequeF
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids276 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity
- 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-deazaguanine + 2 NADPH + 3 H+
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 83-85 | substrate | |||
Binding site | 85-86 | NADPH (UniProtKB | ChEBI) | |||
Active site | 184 | Thioimide intermediate | |||
Active site | 191 | Proton donor | |||
Binding site | 223-224 | substrate | |||
Binding site | 252-253 | NADPH (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | preQ1 synthase activity | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent 7-cyano-7-deazaguanine reductase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionA0A172YYU0
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 16-126 | NADPH-dependent 7-cyano-7-deazaguanine reductase N-terminal | |||
Sequence similarities
Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length276
- Mass (Da)30,886
- Last updated2016-09-07 v1
- MD5 Checksum4BDEF18B6ED62388D3E5425C9B509228
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP015600 EMBL· GenBank· DDBJ | ANF85126.1 EMBL· GenBank· DDBJ | Genomic DNA |