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A0A172YV03 · A0A172YV03_9PSED

  • Protein
    NH(3)-dependent NAD(+) synthetase
  • Gene
    nadE
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (ammonia route): step 1/1.

Features

Showing features for binding site.

127520406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site50-57ATP (UniProtKB | ChEBI)
Binding site56Mg2+ (UniProtKB | ChEBI)
Binding site147deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site167ATP (UniProtKB | ChEBI)
Binding site172Mg2+ (UniProtKB | ChEBI)
Binding site180deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site187deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site196ATP (UniProtKB | ChEBI)
Binding site218ATP (UniProtKB | ChEBI)
Binding site267-268deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular Functionmetal ion binding
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NH(3)-dependent NAD(+) synthetase
  • EC number

Gene names

    • Name
      nadE
    • ORF names
      A7J50_0531

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • PAMC 27494
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0A172YV03

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain28-272NAD/GMP synthase

Sequence similarities

Belongs to the NAD synthetase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    275
  • Mass (Da)
    29,778
  • Last updated
    2016-09-07 v1
  • MD5 Checksum
    A80CFB16EEBDB83ED5D750D474BA2908
MQAVQREIAQQLKVQAPFKDQAALEAEVARRVTFIQDCLRNSGLKTLVLGISGGVDSLTAGLLAQRAMQELRASTGDDAYRFIAVRLPYETQFDEIDAQASVDFIEPDERHTVNIGPAVKSLASEVAAFEGKAAVSRDFVLGNTKARMRMVAQYTIAGAASGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIARHFGAPESLVEKIPTADLEDLSPGKPDEASHGVTYAEIDAFLHGEPVREEAFRIICDTYRKTEHKRVMPFAP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP015600
EMBL· GenBank· DDBJ
ANF83978.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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