A0A166YZY9 · PPZC_METRR

Function

function

2-oxoglutarate-Fe(II) type oxidoreductase; part of the gene cluster that mediates the biosynthesis of pyrrolopyrazines, secondary metabolites showing insecticidal activity (PubMed:30452111).
The single multifunctional NRPS ppzA is responsible for the biosynthesis of peramine (PubMed:30452111).
The condensation domain of ppzA is proposed to catalyze formation of a peptide bond between 1-pyrroline-5-carboxylate and arginine (By similarity).
The methylation domain of ppzA would catalyze the N-methylation of the alpha-amino group of arginine (By similarity).
The reductase domain is proposed to be responsible for reduction of the thioester and the cyclization to form an iminium ion resulting in release from the peptide synthetase (By similarity).
Deprotonation of this intermediate and oxidation of the pyrroline ring would give rise to peramine (By similarity).
This final oxidation to give the pyrrole functionality may be spontaneous (By similarity).
In Epichloe species that produce only peramine, the peramine synthetase gene is not localized in a gene cluster, in contrast to Metarhizium rileyi that contains additional pyrrolopyrazine biosynthesis genes (Probable). The 2-oxoglutarate-Fe(II) type oxidoreductases ppzC and ppzD could be candidates for conversion of proline into an oxidized derivative to be used by the ppzA A1-domain as substrate (Probable). The other ppz genes encode proteins predicted to derivatize peramine into more complex pyrrolopyrazine metabolites (Probable)

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site254Fe cation (UniProtKB | ChEBI)
Binding site256Fe cation (UniProtKB | ChEBI)
Binding site311Fe cation (UniProtKB | ChEBI)
Binding site3212-oxoglutarate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functiondioxygenase activity
Molecular Functionmetal ion binding
Biological Processsmall molecule biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-oxoglutarate-Fe(II) type oxidoreductase ppzC
  • EC number
  • Alternative names
    • Pyrrolopyrazine biosynthesis cluster protein C

Gene names

    • Name
      ppzC
    • ORF names
      NOR_07097

Organism names

Accessions

  • Primary accession
    A0A166YZY9

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004502571-3742-oxoglutarate-Fe(II) type oxidoreductase ppzC

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region111-130Disordered
Domain220-330Fe2OG dioxygenase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    374
  • Mass (Da)
    41,480
  • Last updated
    2016-07-06 v1
  • Checksum
    4B8FFDD0AB8B09F3
MATDTAVTGAPETTELRLASGNGPVTRTVLRTPVRDALPSEVPLIDISPIFSTCVADRKAVARKIHDAATNIGFFYIQNHRVPSQDIDLAYSASQDFFRQEMEVKVEADAKDGPFDSGYRGPGTQRVNPTEGADLRETYSILYDPMLDPTVPDPANIPEPASRFLHLGRAPFESTATLPHFKDAFVRYFQACLVLARALTRAFALSLDLPESAFDGKVQYPDASLEINFYPPISTGHAVSAPGDADTRVSIGSHTDFLLFTILWQDSNGGLQVLNREGQWIRAIPVEGTFVVNIGDYLQRVTNDKYVSTVHRAQNFSGRERVSMPFFWGFGMHESCQVLRNCCGEDEKSKYDEVKCVDWVSRRLGNLFDLSDKG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AZHC01000030
EMBL· GenBank· DDBJ
OAA37398.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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