A0A166YZU9 · PPZE_METRR

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of pyrrolopyrazines, secondary metabolites showing insecticidal activity (PubMed:30452111).
The single multifunctional NRPS ppzA is responsible for the biosynthesis of peramine (PubMed:30452111).
The condensation domain of ppzA is proposed to catalyze formation of a peptide bond between 1-pyrroline-5-carboxylate and arginine (By similarity).
The methylation domain of ppzA would catalyze the N-methylation of the alpha-amino group of arginine (By similarity).
The reductase domain is proposed to be responsible for reduction of the thioester and the cyclization to form an iminium ion resulting in release from the peptide synthetase (By similarity).
Deprotonation of this intermediate and oxidation of the pyrroline ring would give rise to peramine (By similarity).
This final oxidation to give the pyrrole functionality may be spontaneous (By similarity).
In Epichloe species that produce only peramine, the peramine synthetase gene is not localized in a gene cluster, in contrast to Metarhizium rileyi that contains additional pyrrolopyrazine biosynthesis genes (Probable). The 2-oxoglutarate-Fe(II) type oxidoreductases ppzC and ppzD could be candidates for conversion of proline into an oxidized derivative to be used by the ppzA A1-domain as substrate (Probable). The other ppz genes encode proteins predicted to derivatize peramine into more complex pyrrolopyrazine metabolites (Probable)

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site353Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase ppzE
  • EC number
  • Alternative names
    • Pyrrolopyrazine biosynthesis cluster protein E

Gene names

    • Name
      ppzE
    • ORF names
      NOR_07094

Organism names

Accessions

  • Primary accession
    A0A166YZU9

Proteomes

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane10-30Helical

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00004502631-392Cytochrome P450 monooxygenase ppzE
Glycosylation319N-linked (GlcNAc...) asparagine
Glycosylation372N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    392
  • Mass (Da)
    44,641
  • Last updated
    2016-07-06 v1
  • Checksum
    7AAF7E1ECA7CDBC5
MLSIIHVGWLELVWFVALYPFACWTLFAVLKSVYRITLHPLAKFPGPKLAGASYCYEFWYEIVCGIQYTQKIIKLHEQYGPIVRINPDELHFNDIDFVDVVYTAGARKRDKSRHYLAGFEGSIIRFVSSDFHSFLIDTRVKKPERLKRVVLGAERHHDAKDCPMFLELLNSNLPAQEKSKQRLMYEANGATLAGSGSTAIALSNIVYNLVANPRIGHKLRSELMRKVSASKNLPTWSTLEELPYLTAVIHEGLRSMYDPSKERLPYDPSQERLPRVATEEELIYEGGSALGKSKYVIPRGYAISTSAHVVHSDESIFPNASQFDPERWLDRDGQRNKELERHLLSFSKGSRHCLGMHCRRATCLAIPASARNGTSGYQFTSGQLEYKVQERQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AZHC01000030
EMBL· GenBank· DDBJ
OAA37395.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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