A0A166PMR4 · A0A166PMR4_PSEFL

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

TypeIDPosition(s)Description
Site33Interaction with DNA
Site175Interaction with DNA
Site176Interaction with DNA
Site179Interaction with DNA
Site184Interaction with DNA
Site191Interaction with DNA
Active site326O-(5'-phospho-DNA)-tyrosine intermediate
Site328Interaction with DNA
Site514Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      A1D17_24150

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • FW300-N1B4
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0A166PMR4

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain3-149Toprim
Region37-65Disordered
Region199-204Interaction with DNA
Region366-389Disordered

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    875
  • Mass (Da)
    97,310
  • Last updated
    2016-07-06 v1
  • Checksum
    8F4233BED6066EDC
MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASASKEPAAKRGKAAAGEGPVLTPKEKARKQLVSRMGVDPDHGWKAKYEILPGKEKVIEELRRLAKDADTIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSKPGELDIDRVNAQQARRFLDRVVGYMVSPLLWAKVARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEVHADLGTAKGATVRFDVAREKGEAFKPLNEAQAMAALEKLKASSYSIVKREDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSADAVAMARTYIEGEFGKKYLPENPNVYSSKEGAQEAHEAIRPSDANTEPSKLSGMERDAERLYELIWRQFLACQMLPAQYLSTTVTVGAGDFELRAKGRILKFDGYTRVMPQIAKPGDDDVLPDMAQGDAMKLIKLDPTQHFTKPPARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVALHNRRFYSEKMGDIVTERLAESFSNLMDYGFTAGMEENLDDVAQGERDWKNVLDEFYGDFKKKLEVAESPEGGMRANQPVMTDIPCLLCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVNLVPGDEIAADDEGESESLVLRGKHRCPICSTAMDAYLLDEKRKLHICGNNPDCSGYEIEEGTYRIKGYEGPSLECDKCGSEMQLKTGRFGKFFGCTNATCKNTRKLLKSGDAAPPKMDPVKMPELKCEKVNDTYILRDGASGLFLAASQFPKNRETRAPLVMEIVPHKDEIDPKYHFLCEAPKKDPDGLPSVIRYSRKTKEQYVQTEVDGKPTGWKAYYDGGKWTVEDKRPSAKA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LUKJ01000003
EMBL· GenBank· DDBJ
KZN19108.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp