A0A162I360 · A0A162I360_METRR
- ProteinDNA replication ATP-dependent helicase/nuclease
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1529 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 5'-flap endonuclease activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | single-stranded DNA helicase activity | |
Biological Process | DNA repair | |
Biological Process | DNA replication, Okazaki fragment processing | |
Biological Process | replication fork reversal |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA replication ATP-dependent helicase/nuclease
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Clavicipitaceae > Metarhizium
Accessions
- Primary accessionA0A162I360
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-27 | Polar residues | |||
Region | 1-76 | Disordered | |||
Region | 125-156 | Disordered | |||
Region | 228-262 | Disordered | |||
Compositional bias | 245-262 | Polar residues | |||
Region | 275-329 | Disordered | |||
Compositional bias | 290-305 | Basic and acidic residues | |||
Compositional bias | 306-322 | Polar residues | |||
Domain | 451-653 | DNA replication factor Dna2 N-terminal | |||
Domain | 661-763 | DUF83 | |||
Domain | 825-916 | DNA2 rift barrel | |||
Domain | 1016-1104 | DNA2/NAM7 helicase helicase | |||
Domain | 1118-1184 | DNA2/NAM7 helicase helicase | |||
Domain | 1193-1421 | DNA2/NAM7 helicase-like C-terminal | |||
Region | 1460-1502 | Disordered | |||
Sequence similarities
Belongs to the DNA2/NAM7 helicase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,529
- Mass (Da)170,885
- Last updated2016-07-06 v1
- Checksum8661ADDFFA940193
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-27 | Polar residues | |||
Compositional bias | 245-262 | Polar residues | |||
Compositional bias | 290-305 | Basic and acidic residues | |||
Compositional bias | 306-322 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AZHC01000001 EMBL· GenBank· DDBJ | OAA51715.1 EMBL· GenBank· DDBJ | Genomic DNA |