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A0A162BM99 · A0A162BM99_9ENTR

  • Protein
    Dual-specificity RNA methyltransferase RlmN
  • Gene
    rlmN
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site109Proton acceptor
Binding site129[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site133[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site136[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site183-184S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site215S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site237-239S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site316S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site359S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • Synonyms
      trmG/rlmN
    • ORF names
      ABF77_04695
      , AYL25_17810
      , HNS33_16845
      , J0A64_15005
      , OIPHN260_35120
      , SAMEA2273443_02835

Organism names

  • Taxonomic identifier
  • Strains
    • GN02692
    • E2161
    • e2161
    • PDA134
    • YK16
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Enterobacter > Enterobacter cloacae complex

Accessions

  • Primary accession
    A0A162BM99
  • Secondary accessions
    • A0A0W2HA39

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond122↔359(transient)

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain115-354Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    388
  • Mass (Da)
    43,438
  • Last updated
    2016-07-06 v1
  • MD5 Checksum
    CF38C79A30902A47C948CE32F7BDAF59
MSELVNTSEVAIPAVPNKNGKINLLDLNRQQMREFFKEMGEKPFRADQVMKWMYHYCSDNFDDMTDINKVLRNKLKEVAEIRAPEVVEEQRSADGTIKWAIAVGDQRVETVYIPEEDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGTRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGETIAVKAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP023447
EMBL· GenBank· DDBJ
BCL44010.1
EMBL· GenBank· DDBJ
Genomic DNA
LEDI01000008
EMBL· GenBank· DDBJ
KLQ06783.1
EMBL· GenBank· DDBJ
Genomic DNA
JABJWE010000011
EMBL· GenBank· DDBJ
MBA2154522.1
EMBL· GenBank· DDBJ
Genomic DNA
JAFKCP010000009
EMBL· GenBank· DDBJ
MBU3767908.1
EMBL· GenBank· DDBJ
Genomic DNA
LSQV01000026
EMBL· GenBank· DDBJ
OBS87611.1
EMBL· GenBank· DDBJ
Genomic DNA
FKDK01000011
EMBL· GenBank· DDBJ
SAA76711.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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