A0A157BZ56 · A0A157BZ56_ENTCL
- ProteinAdenylosuccinate synthetase
- GenepurA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids446 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
Catalytic activity
- IMP + L-aspartate + GTP = N6-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H+
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 27-33 | GTP (UniProtKB | ChEBI) | |||
Active site | 28 | Proton acceptor | |||
Binding site | 28 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 28-31 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 53-56 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 55 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 55-57 | GTP (UniProtKB | ChEBI) | |||
Active site | 56 | Proton donor | |||
Binding site | 144 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Active site | 155 | ||||
Binding site | 158 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 239 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 254 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 314-320 | substrate | |||
Binding site | 318 | IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 320 | GTP (UniProtKB | ChEBI) | |||
Binding site | 346-348 | GTP (UniProtKB | ChEBI) | |||
Binding site | 429-431 | GTP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenylosuccinate synthase activity | |
Molecular Function | GTP binding | |
Molecular Function | magnesium ion binding | |
Biological Process | 'de novo' AMP biosynthetic process | |
Biological Process | IMP metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenylosuccinate synthetase
- EC number
- Short namesAMPSase ; AdSS
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Enterobacter > Enterobacter cloacae complex
Accessions
- Primary accessionA0A157BZ56
- Secondary accessions
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length446
- Mass (Da)48,870
- Last updated2016-06-08 v1
- Checksum702171757BE3B54E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP022126 EMBL· GenBank· DDBJ | BBS30247.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGJB01000004 EMBL· GenBank· DDBJ | STQ14648.1 EMBL· GenBank· DDBJ | Genomic DNA |