A0A157BZ56 · A0A157BZ56_ENTCL

  • Protein
    Adenylosuccinate synthetase
  • Gene
    purA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site27-33GTP (UniProtKB | ChEBI)
Active site28Proton acceptor
Binding site28Mg2+ (UniProtKB | ChEBI)
Binding site28-31IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site53-56IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site55Mg2+ (UniProtKB | ChEBI)
Binding site55-57GTP (UniProtKB | ChEBI)
Active site56Proton donor
Binding site144IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Active site155
Binding site158IMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site239IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site254IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site314-320substrate
Binding site318IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site320GTP (UniProtKB | ChEBI)
Binding site346-348GTP (UniProtKB | ChEBI)
Binding site429-431GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionadenylosuccinate synthase activity
Molecular FunctionGTP binding
Molecular Functionmagnesium ion binding
Biological Process'de novo' AMP biosynthetic process
Biological ProcessIMP metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Adenylosuccinate synthetase
  • EC number
  • Short names
    AMPSase
    ; AdSS
  • Alternative names
    • IMP--aspartate ligase

Gene names

    • Name
      purA
    • ORF names
      NCTC10005_07513
      , WP5S18C02_04530

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • NCTC10005
    • WP5-S18-CRE-02
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Enterobacter > Enterobacter cloacae complex

Accessions

  • Primary accession
    A0A157BZ56
  • Secondary accessions
    • A0A0M7F0Y5

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the adenylosuccinate synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    446
  • Mass (Da)
    48,870
  • Last updated
    2016-06-08 v1
  • Checksum
    702171757BE3B54E
MVESIFKQTVILKKMGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKGLEDRGIPVRERLLLSEACPLILDYHVALDVAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKATFAEKLKEVMEYHNFQLVNFYKADAVDYQKVLDDVMAIADILTGMVVDVSDLLDQARKRGDFVMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGIIKAYSTRVGAGPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDAVAVRRAVQINSLSGFCLTKLDVLDGLKEVKICVGYRMPDGREVTTTPLAADDWEGIEPIYETMPGWSETTFGVKERSGLPQAALDYIKRIEELTEVPIDIISTGPDRTETMILRDPFDA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP022126
EMBL· GenBank· DDBJ
BBS30247.1
EMBL· GenBank· DDBJ
Genomic DNA
UGJB01000004
EMBL· GenBank· DDBJ
STQ14648.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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