A0A140E064 · A0A140E064_CVHOC
- ProteinHemagglutinin-esterase
- GeneHE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids423 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
Catalytic activity
- N-acetyl-4-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 40 | Nucleophile | ||||
Sequence: S | ||||||
Active site | 325 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 328 | Charge relay system | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell plasma membrane | |
Cellular Component | plasma membrane | |
Cellular Component | viral envelope | |
Cellular Component | virion membrane | |
Molecular Function | host cell surface receptor binding | |
Molecular Function | sialate 4-O-acetylesterase activity | |
Molecular Function | sialate 9-O-acetylesterase activity | |
Biological Process | fusion of virus membrane with host plasma membrane |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHemagglutinin-esterase
- EC number
- Short namesHE protein
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Betacoronavirus > Embecovirus > Betacoronavirus 1
- Virus hosts
Accessions
- Primary accessionA0A140E064
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host cell membrane ; Single-pass type I membrane protein
Virion membrane ; Single-pass type I membrane protein
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.
Features
Showing features for transmembrane, topological domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 391-414 | Helical | ||||
Sequence: VILLGILLGVAIVIIVVLFLHFMV | ||||||
Topological domain | 413-423 | Intravirion | ||||
Sequence: MVDNVTRLHDA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_5023313030 | 17-423 | Hemagglutinin-esterase | |||
Sequence: LGFHNPPTNVVSHVNGDWFLFGDSRSDCNHIVNINPHNYSYMDLNPALCDSGKISSKADNSIFRSFHFTDFYNYTGEGQQIIFYEGVNFTPYHAFKCNRSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVYNGSAQATAFCKSGSLVLNNPVYIAPQANSGIITKVEADFYLSGCDEYIVPLCIFNGKFLSNTKYYDHSQYYFNKDTGVIYGLNSTETITTGFDLNCHYLVLPSGNYLSISNELLLTVPTKAICFNKRKDFTPVQVVDSRWNNARQSDNMTAVACQPPYCYFRNSTTNYVGVYDINHGDAGFTSILSGLLYNSSCFSQQGVFRYDNISSVWPLYPYGRCPTAADINIPDLPICVYDPLPVILLGILLGVAIVIIVVLFLHFMVDNVTRLHDA | ||||||
Disulfide bond | 44↔65 | |||||
Sequence: CNHIVNINPHNYSYMDLNPALC | ||||||
Disulfide bond | 113↔162 | |||||
Sequence: CNRSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVYNGSAQATAFC | ||||||
Disulfide bond | 306↔311 | |||||
Sequence: CQPPYC | ||||||
Disulfide bond | 346↔370 | |||||
Sequence: CFSQQGVFRYDNISSVWPLYPYGRC |
Post-translational modification
N-glycosylated in the host RER.
Keywords
- PTM
Interaction
Subunit
Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 22-376 | Haemagglutinin-esterase glycoprotein core | ||||
Sequence: PPTNVVSHVNGDWFLFGDSRSDCNHIVNINPHNYSYMDLNPALCDSGKISSKADNSIFRSFHFTDFYNYTGEGQQIIFYEGVNFTPYHAFKCNRSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVYNGSAQATAFCKSGSLVLNNPVYIAPQANSGIITKVEADFYLSGCDEYIVPLCIFNGKFLSNTKYYDHSQYYFNKDTGVIYGLNSTETITTGFDLNCHYLVLPSGNYLSISNELLLTVPTKAICFNKRKDFTPVQVVDSRWNNARQSDNMTAVACQPPYCYFRNSTTNYVGVYDINHGDAGFTSILSGLLYNSSCFSQQGVFRYDNISSVWPLYPYGRCPTAADI | ||||||
Domain | 129-262 | Haemagglutinin-esterase glycoprotein haemagglutinin | ||||
Sequence: FYTQVYKNMAVYRSLTFVNVPYVYNGSAQATAFCKSGSLVLNNPVYIAPQANSGIITKVEADFYLSGCDEYIVPLCIFNGKFLSNTKYYDHSQYYFNKDTGVIYGLNSTETITTGFDLNCHYLVLPSGNYLSIS |
Sequence similarities
Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length423
- Mass (Da)47,596
- Last updated2016-05-11 v1
- Checksum8B1D1FDBC004FD5F