A0A136IN16 · A0A136IN16_9PEZI
- ProteintRNA (guanine-N(7)-)-methyltransferase
- GeneTRM8
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1864 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.
Catalytic activity
- guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteine
Pathway
tRNA modification; N7-methylguanine-tRNA biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 103 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 126-127 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: EI | ||||||
Binding site | 161-162 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: NT | ||||||
Binding site | 181 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Active site | 184 | |||||
Sequence: D | ||||||
Binding site | 259-261 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: TEE |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | nucleus | |
Molecular Function | phosphatidylinositol binding | |
Molecular Function | tRNA (guanine(46)-N7)-methyltransferase activity | |
Molecular Function | tRNA binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine-N(7)-)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Xylariomycetidae > Xylariales > Microdochiaceae > Microdochium
Accessions
- Primary accessionA0A136IN16
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 307-325 | Helical | ||||
Sequence: FVIAGIVCFIAWGYAVSWF | ||||||
Transmembrane | 331-352 | Helical | ||||
Sequence: AGYAFASGLTTALIGVLAALVL |
Keywords
- Cellular component
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-45 | Disordered | ||||
Sequence: MAGPPNKKQKREAYRQAQRESGATTMPQKKFYRQRAHANPFSDHQ | ||||||
Region | 66-91 | Disordered | ||||
Sequence: AKPDEGNTTETSRNDPTSLPRDPKNQ | ||||||
Compositional bias | 70-90 | Polar residues | ||||
Sequence: EGNTTETSRNDPTSLPRDPKN | ||||||
Domain | 401-590 | PXA | ||||
Sequence: SPSLSRALDELLGLILRDFVDSWYSRISKNPVFTNEVDRNIRFALSALRDRLAEADLVEAAISRIVPILTDHFRDFFDAERAVRGRKLNRDVTESEELDLAIAAKFRDGKLHAAASLSYADTKIVRQEHLRKMVTRILPQVLPENMITSRAVLSIVREIVSCAVLFPVMQVLSEPDTWNQIMENYGRSML | ||||||
Region | 691-710 | Disordered | ||||
Sequence: AGGDRHGTNPTQLSAPSLES | ||||||
Domain | 717-856 | RGS | ||||
Sequence: SLAELLRDTSGLSYFMEFMDRQGMMTHVQFWLVVDGFRNPLEDDGPEDAELPSTTVPWTDSDRTDLAQIDQAYLTKPELRVPVSSRQAIREFLAAGKAATNKQYYAARRSVLRAQSTVLEEMETRYLQAFKKSDLFFKAL | ||||||
Compositional bias | 872-898 | Polar residues | ||||
Sequence: AAPELHRASQSFTTKPTVGSTKANPRL | ||||||
Region | 872-901 | Disordered | ||||
Sequence: AAPELHRASQSFTTKPTVGSTKANPRLKAV | ||||||
Compositional bias | 913-927 | Polar residues | ||||
Sequence: ELGQANSSSSSMDPL | ||||||
Region | 913-959 | Disordered | ||||
Sequence: ELGQANSSSSSMDPLHGPRRSLDADARTHVRQNSEDSSHTPLFDDDD | ||||||
Compositional bias | 930-959 | Basic and acidic residues | ||||
Sequence: PRRSLDADARTHVRQNSEDSSHTPLFDDDD | ||||||
Region | 1018-1046 | Disordered | ||||
Sequence: AQPSLFSGLEQDNASLENSRVLPDGKEGD | ||||||
Coiled coil | 1144-1171 | |||||
Sequence: LRILRKSRASLQRELRRKELQRQQYVIQ | ||||||
Domain | 1180-1298 | PX | ||||
Sequence: KASVQIKSIQVGKDEDGKEFAVYVVEVQRRAGEQMPAATWIVTRRYSEFHDLHQRLRLRYPSVRHLDFPRRRMVMKLQSDFLRKRSQALEQYLKELLQLPDVCRSRDLRSFLSQSVITQ | ||||||
Domain | 1560-1621 | RRM | ||||
Sequence: TTKTDLETHFATHGTGEITEIKLMNGFGFVEYKDPMDARDVVPAFHGSDFMGQRLTVQFARG | ||||||
Region | 1623-1657 | Disordered | ||||
Sequence: RQREAGFGGNNERQPPRPRRTPHRMQITGLPNDTS | ||||||
Domain | 1645-1712 | RRM | ||||
Sequence: HRMQITGLPNDTSWQDLKDFARQSSLDVVFSETGRDGNGRGFVEFESAADLRTAIEKLDQREFKGQRV | ||||||
Region | 1713-1864 | Disordered | ||||
Sequence: TCIADTQPDMPFRDRGRSRSPGFRKPYGMPRDPYDRRGPPPTGYSPPRGGYREDYRDRSPRREYYDDRRYRSPPRRGPPMEEYGAPPRRYDDPYRRDYGPPPADPYAAGRGYDRPPREFPPREPGYPPRDPGGYPPHPREDYRRGNGGPPGY | ||||||
Compositional bias | 1762-1788 | Basic and acidic residues | ||||
Sequence: GYREDYRDRSPRREYYDDRRYRSPPRR |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
Belongs to the sorting nexin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,864
- Mass (Da)210,832
- Last updated2016-05-11 v1
- ChecksumA8E35F50D59EF86B
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 70-90 | Polar residues | ||||
Sequence: EGNTTETSRNDPTSLPRDPKN | ||||||
Compositional bias | 872-898 | Polar residues | ||||
Sequence: AAPELHRASQSFTTKPTVGSTKANPRL | ||||||
Compositional bias | 913-927 | Polar residues | ||||
Sequence: ELGQANSSSSSMDPL | ||||||
Compositional bias | 930-959 | Basic and acidic residues | ||||
Sequence: PRRSLDADARTHVRQNSEDSSHTPLFDDDD | ||||||
Compositional bias | 1762-1788 | Basic and acidic residues | ||||
Sequence: GYREDYRDRSPRREYYDDRRYRSPPRR |
Keywords
- Technical term