A0A135NYS8 · A0A135NYS8_9HYPH
- ProteinElongation factor 4
- GenelepA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids612 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.
Catalytic activity
- GTP + H2O = GDP + phosphate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | guanosine tetraphosphate binding | |
Molecular Function | ribosome binding | |
Molecular Function | translation elongation factor activity | |
Biological Process | positive regulation of translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameElongation factor 4
- EC number
- Short namesEF-4
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Agrobacterium
Accessions
- Primary accessionA0A135NYS8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-197 | Tr-type G | ||||
Sequence: DHIRNFSIVAHIDHGKSTLADRLIQSTGGLAERDMSEQVLDNMDIERERGITIKAQTVRLHYKANNGEIYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNDHEIVTVLNKIDLPAAEPDRIREQIEEVIGIDASQAVLISAKTGLGIPDVLEAIVHQLPPPK |
Sequence similarities
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length612
- Mass (Da)67,684
- Last updated2016-06-08 v1
- ChecksumA1239DBC98CD3097
Keywords
- Technical term