A0A125YSC7 · FNT1_TOXGM
- ProteinFormate-nitrite transporter 1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids412 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Monocarboxylate-proton symporter; active in acidic-to-neutral pH range (PubMed:30237165).
Transports L-lactate and formate (PubMed:30237165, PubMed:33762657).
Probably acts as a primary L-lactate transporter in extracellular tachyzoites (PubMed:33762657).
Transports L-lactate and formate (PubMed:30237165, PubMed:33762657).
Probably acts as a primary L-lactate transporter in extracellular tachyzoites (PubMed:33762657).
Miscellaneous
Not sensitive to p-chloromercuribenzene sulfonate (pCMBS) (PubMed:30237165).
Inhibitors modestly slow the asexual reproduction of parasites (PubMed:30237165).
Frameshift mutations that are likely to render the resulting proteins non-functional, have no significant effects on tachyzoite proliferation in vitro (PubMed:33762657).
Inhibitors modestly slow the asexual reproduction of parasites (PubMed:30237165).
Frameshift mutations that are likely to render the resulting proteins non-functional, have no significant effects on tachyzoite proliferation in vitro (PubMed:33762657).
Catalytic activity
- (S)-lactate(in) + H+(in) = (S)-lactate(out) + H+(out)(S)-lactate (in)CHEBI:16651
+ H+ (in)CHEBI:15378= (S)-lactate (out)CHEBI:16651+ H+ (out)CHEBI:15378 - formate(in) + H+(in) = formate(out) + H+(out)
- pyruvate(out) + H+(out) = pyruvate(in) + H+(in)
- acetate(out) + H+(out) = acetate(in) + H+(in)
Activity regulation
Inhibited by methyl methanethiosulfonate (MMTS) (PubMed:30237165).
Partially inhibited by the Malaria Box compound MMV007839 (PubMed:30237165).
Partially inhibited by BH-296, BH-317, BH-326 and BH-388 compounds (PubMed:30237165).
Partially inhibited by the Malaria Box compound MMV007839 (PubMed:30237165).
Partially inhibited by BH-296, BH-317, BH-326 and BH-388 compounds (PubMed:30237165).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | high-affinity secondary active nitrite transmembrane transporter activity | |
Biological Process | nitrite transport |
Names & Taxonomy
Protein names
- Recommended nameFormate-nitrite transporter 1
- Short namesTgFNT1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Conoidasida > Coccidia > Eucoccidiorida > Eimeriorina > Sarcocystidae > Toxoplasma
Accessions
- Primary accessionA0A125YSC7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-32 | Cytoplasmic | |||
Transmembrane | 33-53 | Helical | |||
Topological domain | 54-69 | Extracellular | |||
Transmembrane | 70-90 | Helical | |||
Topological domain | 91-116 | Cytoplasmic | |||
Transmembrane | 117-137 | Helical | |||
Topological domain | 138-164 | Extracellular | |||
Transmembrane | 165-185 | Helical | |||
Topological domain | 186-192 | Cytoplasmic | |||
Transmembrane | 193-213 | Helical | |||
Topological domain | 214-237 | Extracellular | |||
Transmembrane | 238-258 | Helical | |||
Topological domain | 259-412 | Cytoplasmic | |||
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 93 | Reduces lactate transport. Reduces sensitivity to MMV007839 inhibition. | |||
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000461324 | 1-412 | Formate-nitrite transporter 1 | ||
Glycosylation | 151 | N-linked (GlcNAc...) asparagine | |||
Keywords
- PTM
Interaction
Subunit
Homopentamer.
Structure
Sequence
- Sequence statusComplete
- Length412
- Mass (Da)44,290
- Last updated2016-04-13 v1
- MD5 Checksum96F9A3DB1A8F4043073001F6D31DD14C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KE138815 EMBL· GenBank· DDBJ | EPT32283.1 EMBL· GenBank· DDBJ | Genomic DNA |