A0A125R003 · CLA3_CLACD
- ProteinNon-reducing polyketide synthase cla3
- Genecla3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids2073 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Highly reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of cladosporin, a tricyclic octaketide that acts as an antimalarial agent though inhibition of the Plasmodium falciparum lysyl-tRNA synthetase (PubMed:26783060).
The highly reducing polyketide synthase cla2 is responsible for biosynthesis up to the pentaketide stage, including of the tetrahydropyran (THP) ring, whereas the three subsequent ketide extensions with no reduction are catalyzed by the non-reducing polyketide synthase cla3 (PubMed:26783060).
The highly reducing polyketide synthase cla2 is responsible for biosynthesis up to the pentaketide stage, including of the tetrahydropyran (THP) ring, whereas the three subsequent ketide extensions with no reduction are catalyzed by the non-reducing polyketide synthase cla3 (PubMed:26783060).
Biotechnology
Cladosporin has been intensely studied for its antimalarial activity though inhibition of the Plasmodium falciparum lysyl-tRNA synthetase (PubMed:22704625, PubMed:24935905, PubMed:26074468).
Cladosporin has also antifungal activity against dermatophytes, as well as Penicillium and Aspergillus species (PubMed:5169000).
Cladosporin has also antifungal activity against dermatophytes, as well as Penicillium and Aspergillus species (PubMed:5169000).
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 538 | For beta-ketoacyl synthase activity | |||
Active site | 673 | For beta-ketoacyl synthase activity | |||
Active site | 712 | For beta-ketoacyl synthase activity | |||
Active site | 987 | For acyl/malonyl transferase activity | |||
Active site | 1311 | Proton acceptor; for dehydratase activity | |||
Active site | 1500 | Proton donor; for dehydratase activity | |||
Active site | 2058 | For thioesterase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameNon-reducing polyketide synthase cla3
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Dothideomycetidae > Cladosporiales > Cladosporiaceae > Cladosporium
Accessions
- Primary accessionA0A125R003
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000437052 | 1-2073 | Non-reducing polyketide synthase cla3 | ||
Modified residue | 1678 | O-(pantetheine 4'-phosphoryl)serine | |||
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 9-242 | N-terminal acylcarrier protein transacylase domain (SAT) | |||
Domain | 363-793 | Ketosynthase family 3 (KS3) | |||
Region | 898-1198 | Malonyl-CoA:ACP transacylase (MAT) domain | |||
Region | 1276-1590 | Product template (PT) domain | |||
Region | 1279-1420 | N-terminal hotdog fold | |||
Domain | 1279-1586 | PKS/mFAS DH | |||
Region | 1439-1586 | C-terminal hotdog fold | |||
Region | 1594-1637 | Disordered | |||
Domain | 1641-1718 | Carrier | |||
Region | 1721-1786 | Disordered | |||
Compositional bias | 1723-1741 | Polar residues | |||
Compositional bias | 1743-1771 | Basic and acidic residues | |||
Region | 1805-1950 | Thioesterase (TE) domain | |||
Domain
Multidomain protein; including a starter unit:ACP transacylase (SAT) that selects the starter unit; a ketosynthase (KS) that catalyzes repeated decarboxylative condensation to elongate the polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects and transfers the extender unit malonyl-CoA; a product template (PT) domain that controls the immediate cyclization regioselectivity of the reactive polyketide backbone; and an acyl-carrier protein (ACP) that serves as the tether of the growing and completed polyketide via its phosphopantetheinyl arm (By similarity).
The release of the polyketide chain from the non-reducing polyketide synthase is mediated by the thioesterase (TE) domain localized at the C-ter of the protein (By similarity).
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,073
- Mass (Da)225,592
- Last updated2016-04-13 v1
- MD5 Checksum9CFF90A167F3E693CB1C7B34B230A8E4
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1723-1741 | Polar residues | |||
Compositional bias | 1743-1771 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KT037692 EMBL· GenBank· DDBJ | AMB51800.1 EMBL· GenBank· DDBJ | mRNA |