A0A117EFX0 · A0A117EFX0_STRSC
- ProteinAminodeoxyfutalosine synthase
- GenemqnE_1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids387 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.
Catalytic activity
- 3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L-methionine = 6-amino-6-deoxyfutalosine + H+ + hydrogencarbonate + L-methionine
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Quinol/quinone metabolism; menaquinone biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | aminodeoxyfutalosine synthase activity | |
Molecular Function | iron ion binding | |
Biological Process | menaquinone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAminodeoxyfutalosine synthase
- EC number
- Short namesAFL synthase ; Aminofutalosine synthase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionA0A117EFX0
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 52-279 | Radical SAM core | ||||
Sequence: VHFNVNRHLNMTNVCTASCAYCSFQRKPGEKDAYTMRIEEAVKLAKAMEGENLTELHIVNGLHPNLPWRYYPRSLKELKAALPNVSLKAFTATEIHHFETISGLTASEILDELIDAGLESLTGGGAEIFDWEVRQHIVDHRTHWEDWSRIHRLAHEKGLKTPCTMLYGHIEEPRHRVDHVLRLRELQDETGGFQVFIPLRYQHDFVDMQDGKVRNRLQARTQMATGAE |
Sequence similarities
Belongs to the radical SAM superfamily. MqnE family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length387
- Mass (Da)44,417
- Last updated2016-04-13 v1
- ChecksumE07BE660A2EB2714
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BCMM01000038 EMBL· GenBank· DDBJ | GAQ66213.1 EMBL· GenBank· DDBJ | Genomic DNA |