A0A109QY04 · A0A109QY04_9MICO

  • Protein
    Bifunctional enzyme IspD/IspF
  • Gene
    ispDF
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for site, binding site.

Type
IDPosition(s)Description
Site22Transition state stabilizer
Site29Transition state stabilizer
Site164Positions MEP for the nucleophilic attack
Site217Positions MEP for the nucleophilic attack
Binding site248a divalent metal cation (UniProtKB | ChEBI)
Binding site248-2504-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site250a divalent metal cation (UniProtKB | ChEBI)
Site282Transition state stabilizer
Binding site282-2834-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site290a divalent metal cation (UniProtKB | ChEBI)
Binding site304-3064-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site377-3804-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site378Transition state stabilizer
Binding site3844-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site3874-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional enzyme IspD/IspF

Including 2 domains:

  • Recommended name
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • EC number
  • Alternative names
    • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    • MEP cytidylyltransferase
      (MCT
      )
  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispDF
    • ORF names
      AWU67_10980

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ERGS5:02
  • Taxonomic lineage
    Bacteria > Bacillati > Actinomycetota > Actinomycetes > Micrococcales > Microbacteriaceae > Microterricola

Accessions

  • Primary accession
    A0A109QY04

Proteomes

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-2412-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Domain242-3992-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region242-4092-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
In the C-terminal section; belongs to the IspF family.
In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    409
  • Mass (Da)
    40,901
  • Last updated
    2016-04-13 v1
  • MD5 Checksum
    B80DA3AD9D7C1C72BF21D850FBE64A28
MDFSGDSVDVAVIVVAAGSGTRLGRPEPKAFAPLAGRPMLSHALDGIFTLAEPVQLIVVAPDAELQSARAIVTASAGPAIGYTSVVAGGATRQLSVAAGLAAVAPGVTVVLVHDAARALTPAAQFEAVIAAVRATGSGVIPALPVVDTIKRVDAAGTVQETVDRAELAAVQTPQGFVRSELDEAYADPAADFTDDAALLAAFGHPVGTVAGDELAFKITTPWDLARAERLLAAPAAAASAPRVGTGMDVHAFAPGSADAADAGPELWIAGLYWPGQRGLSGHSDGDVAVHAVCDALLAAAGLGDIGGIFGTDDPRLAGAHGEVFLRETKRLVEQAGFAIGNVSVQILGNRPKLSPRRVEAEALLTGILNAPVSIAATTTDGLGFTGRGEGVAAIATALLVPSAALGEGV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP014145
EMBL· GenBank· DDBJ
AMB59300.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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