A0A106YVZ9 · A0A106YVZ9_BURCE

Function

function

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.

Features

Showing features for site, binding site, active site.

Type
IDPosition(s)Description
Site53Part of a proton relay during catalysis
Binding site54pyruvate (UniProtKB | ChEBI)
Site58L-lysine inhibitor binding; via carbonyl oxygen
Site89L-lysine inhibitor binding
Site93L-lysine inhibitor binding
Site115L-lysine inhibitor binding
Site116Part of a proton relay during catalysis
Active site142Proton donor/acceptor
Active site170Schiff-base intermediate with substrate
Binding site212pyruvate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function4-hydroxy-tetrahydrodipicolinate synthase activity
Biological Processdiaminopimelate biosynthetic process
Biological Processlysine biosynthetic process via diaminopimelate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-tetrahydrodipicolinate synthase
  • EC number
  • Short names
    HTPA synthase

Gene names

    • Name
      dapA_5
    • Synonyms
      dapA
    • ORF names
      E3D37_10390
      , JAO13_14420
      , NCTC10661_05961
      , WS90_21415
      , WT26_14675

Organism names

  • Taxonomic identifier
  • Strains
    • 871
    • MSMB1302
    • MSMB1184WGS
    • NCTC10661
    • D13
    • d13
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Burkholderia > Burkholderia cepacia complex

Accessions

  • Primary accession
    A0A106YVZ9
  • Secondary accessions
    • A0A1I0HQR3

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer; dimer of dimers.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the DapA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    301
  • Mass (Da)
    31,957
  • Last updated
    2016-04-13 v1
  • Checksum
    39063D46FD9D7D69
MANGTQDGIQIRGSIPAIVTPMLEDGSLDLPAFRKLIDWHIAEGTDALVVVGTSGESATLNVEEHILMIRTAVEHAAKRIPIIAGAGGNSTAEAIELTKHAKAVGADATLQVVPYYNKPTQEGMYRHFKTIAEAVDLPVILYNVPGRTVADMANETMLRLAEVPGIIGVKEATGNIDRAAHLIKAAPKHFAIYSGDDPTAIALMLLGGHGNISVTANVAPRAMSELCRAALAGDVQTARELHMKLLSLHKNLFIEANPIPVKWALQAMGKMQGGIRLPLTALDERCHDAVRSALVEAGVLA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP013443
EMBL· GenBank· DDBJ
AOK17132.1
EMBL· GenBank· DDBJ
Genomic DNA
LOYH01000082
EMBL· GenBank· DDBJ
KVK77716.1
EMBL· GenBank· DDBJ
Genomic DNA
JAEDXG010000012
EMBL· GenBank· DDBJ
MBH9697635.1
EMBL· GenBank· DDBJ
Genomic DNA
UARD01000044
EMBL· GenBank· DDBJ
SQA57343.1
EMBL· GenBank· DDBJ
Genomic DNA
SNSQ01000009
EMBL· GenBank· DDBJ
TEU50714.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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