A0A105U261 · A0A105U261_BURCE
- ProteinNADPH-dependent 7-cyano-7-deazaguanine reductase
- GenequeF
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids274 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity
- 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-deazaguanine + 2 NADPH + 3 H+
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 80-82 | substrate | ||||
Sequence: VES | ||||||
Binding site | 82-83 | NADPH (UniProtKB | ChEBI) | ||||
Sequence: SK | ||||||
Active site | 181 | Thioimide intermediate | ||||
Sequence: C | ||||||
Active site | 188 | Proton donor | ||||
Sequence: D | ||||||
Binding site | 220-221 | substrate | ||||
Sequence: HE | ||||||
Binding site | 249-250 | NADPH (UniProtKB | ChEBI) | ||||
Sequence: RG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | preQ1 synthase activity | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent 7-cyano-7-deazaguanine reductase
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Burkholderia > Burkholderia cepacia complex
Accessions
- Primary accessionA0A105U261
- Secondary accessions
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 14-123 | NADPH-dependent 7-cyano-7-deazaguanine reductase N-terminal | ||||
Sequence: YAAQYDASLLFPIPRAGAREQLGIASALPFFGTDIWNAYELSWLNARGKPQIAVATFYVPAESPNIVESKSFKLYLGSFAQTKFDSIDAVRDTLKRDVSAACGASVAVQL |
Sequence similarities
Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length274
- Mass (Da)30,354
- Last updated2016-04-13 v1
- Checksum9C77873AC570FA7F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JAEDXG010000014 EMBL· GenBank· DDBJ | MBH9698051.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
SNSQ01000022 EMBL· GenBank· DDBJ | TEU45183.1 EMBL· GenBank· DDBJ | Genomic DNA |