A0A105U261 · A0A105U261_BURCE

Function

function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site80-82substrate
Binding site82-83NADPH (UniProtKB | ChEBI)
Active site181Thioimide intermediate
Active site188Proton donor
Binding site220-221substrate
Binding site249-250NADPH (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionpreQ1 synthase activity
Biological Processqueuosine biosynthetic process
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent 7-cyano-7-deazaguanine reductase
  • EC number
  • Alternative names
    • 7-cyano-7-carbaguanine reductase
    • NADPH-dependent nitrile oxidoreductase
    • PreQ(0) reductase

Gene names

    • Name
      queF
    • ORF names
      E3D37_19825
      , JAO13_16565

Organism names

  • Taxonomic identifier
  • Strains
    • 871
    • d13
    • D13
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Burkholderia > Burkholderia cepacia complex

Accessions

  • Primary accession
    A0A105U261
  • Secondary accessions
    • A0A1I0LC52

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain14-123NADPH-dependent 7-cyano-7-deazaguanine reductase N-terminal

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    274
  • Mass (Da)
    30,354
  • Last updated
    2016-04-13 v1
  • Checksum
    9C77873AC570FA7F
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGIASALPFFGTDIWNAYELSWLNARGKPQIAVATFYVPAESPNIVESKSFKLYLGSFAQTKFDSIDAVRDTLKRDVSAACGASVAVQLVSPHDFGKLEMDELDGLSLDRLDLDTDVYEPDPSLLSAAVDEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPVKLAVYARYTRRGGLDINPFRTNYNQPMPDNARNARQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAEDXG010000014
EMBL· GenBank· DDBJ
MBH9698051.1
EMBL· GenBank· DDBJ
Genomic DNA
SNSQ01000022
EMBL· GenBank· DDBJ
TEU45183.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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