A0A0W4ZBI4 · A0A0W4ZBI4_PNEC8
- ProteinDNA replication ATP-dependent helicase/nuclease
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1473 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 5'-flap endonuclease activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | single-stranded DNA helicase activity | |
Biological Process | DNA repair | |
Biological Process | DNA replication, Okazaki fragment processing | |
Biological Process | replication fork reversal |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA replication ATP-dependent helicase/nuclease
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Taphrinomycotina > Pneumocystomycetes > Pneumocystaceae > Pneumocystis
Accessions
- Primary accessionA0A0W4ZBI4
Proteomes
Organism-specific databases
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 448-650 | DNA replication factor Dna2 N-terminal | ||||
Sequence: NEENQIILLRQDWFFTPIEIGDYLYLHGEYDENGICIVDNSHSLVILNPDFLVSCTSVATSYSCTRRIILQEKVKLTDDISKSQIYGKILHCLFQLCLENDNFSLSFLKEKTKQLVLDNIEHLDISGESIESAMKHISGKFFNLQKWASKYFLNTQDDNSYIKAFRSRTSNPCHISVNKLLRTEEHILSPKYGLKGYIDVSVQ | ||||||
Domain | 1011-1099 | DNA2/NAM7 helicase helicase | ||||
Sequence: KLNNDQKKAISKVMTALDYTLILGMPGTGKTTTITYLIQFLIANNNSILLASYTHSAIDNILLKLNVEKENVLRLGSIERIHPDLRKKI | ||||||
Domain | 1120-1180 | DNA2/NAM7 helicase helicase | ||||
Sequence: SIVATTCLGITHSIFSKRTFDYCIIDEASQIILPICIGPLRFSKKFVLVGDHYQLPPLVRS | ||||||
Domain | 1189-1404 | DNA2/NAM7 helicase-like C-terminal | ||||
Sequence: EMSLFKHLLDANPSATVNLEYQYRMNKDIMLLSNTLIYNGKLKCGDEKIAYKLFDCGDFQMLDALHSKEGHEFVCNINECWIKKILEPRLSVVFANTDYVPAPESRKGDRIQNEIEVQLLKQVIICLLNQGISESDIAVLSVYRSQIKLIKHHFRQFQKIEIDTADRFQGRDKNCIIISFVRSNDKNEIGELLRDWRRLNVAFTRAKLKLIFFGSK |
Sequence similarities
Belongs to the DNA2/NAM7 helicase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,473
- Mass (Da)171,053
- Last updated2016-03-16 v1
- ChecksumEF1124D751C38761
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LFVZ01000016 EMBL· GenBank· DDBJ | KTW25775.1 EMBL· GenBank· DDBJ | Genomic DNA |