A0A0W0DAZ4 · A0A0W0DAZ4_CANGB

Function

function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, dna binding.

Type
IDPosition(s)Description
Binding site280-287ATP (UniProtKB | ChEBI)
DNA binding745-764

GO annotations

AspectTerm
Cellular Componentmitochondrial membrane
Cellular Componentnuclear replication fork
Cellular Componentsite of double-strand break
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functioncentromeric DNA binding
Molecular FunctionG-quadruplex DNA binding
Molecular Functionsingle-stranded DNA binding
Molecular Functiontelomerase inhibitor activity
Molecular Functiontelomeric DNA binding
Biological ProcessDNA unwinding involved in DNA replication
Biological Processdouble-strand break repair via break-induced replication
Biological ProcessG-quadruplex DNA unwinding
Biological Processmitochondrial genome maintenance
Biological Processnegative regulation of telomere maintenance via telomerase
Biological Processreplication fork reversal
Biological Processtelomere maintenance via recombination
Biological Processtranslesion synthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent DNA helicase PIF1
  • EC number
  • Alternative names
    • DNA repair and recombination helicase PIF1

Gene names

    • Name
      PIF1
    • ORF names
      AO440_004777
      , AO440_005266

Organism names

Accessions

  • Primary accession
    A0A0W0DAZ4

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Interacts with telomerase.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain272-569AAA+ ATPase
Region799-841Disordered

Sequence similarities

Belongs to the helicase family. PIF1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    841
  • Mass (Da)
    95,238
  • Last updated
    2016-03-16 v1
  • Checksum
    0B79FC76DBF82337
MSNKRNSSILRQDDYEINYDELSALLSDSSDWDDLSMDSPQHKRNCQARPIAGANNVEIPRPILRTPKAQLKAPPDCGRSISGLSEIDDSSIIELLQDKKPLEKNQILQNTHVLPRETKPPMLVPLKEIVPKPELNVLENISLMAESTQRAKPQSMNPSSLDISNVDEKVIEKHIEILSPKQVNKELNDLTNSDSSLQINEVLKKDISELSTEEIQFAKAQLGEKFRFLTQRTVFTDVNKKQSRIAAPDTRKVKIPIILSKEQEAVIDLAEKGHNIFYTGSAGTGKSVLLREMIKVLKKKYGPERVAVTASTGLAACNIGGITVHSFAGIGLGNGDVTKLYRKVRRSKKNVKRWSEISVLVIDEISMLDGELFDKLDFIAQKIRKNSDPFGGIQIVLCGDFFQLPPVSKDLSTPMKFAFQSLAWQKAIHVTIMLEKVFRQQGDTKFIDMLNQMRLGKIDMETEMEFKKLNRPLPNDDIIPAELYSTRAEVDRANRSRLNKLPGQAFCYNAIDGGTLEDQEMKDRLLQNFLAPKQLQLKIGAQVMMIKNIDAQLVNGSLGKVIAFMDPETYLFYSTILADSSVTPDVMEEMKDKPEELRERYAGDDEDEKMRVNKKRERFYEGNPNVVPQDDLDDSIFDFMTQATNVSDDARNDIERKKRIIKDLYKNADSRKRLPLVTFKTADMSTRTVLVEPEDWAIEDENEKPLVSRVQLPLMLAWSLSIHKSQGQTLPKVKVDLQRVFEKGQAYVALSRAVSREGLQVLNFDRSRIKAHDTVVDFYMTLVTADQAIEDLKRRNGGSLDKKKAYAPRPAARLDRSQTAPSSGITEMLRRRSRKVETESI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LLZZ01000155
EMBL· GenBank· DDBJ
KTA97920.1
EMBL· GenBank· DDBJ
Genomic DNA
LLZZ01000122
EMBL· GenBank· DDBJ
KTB02965.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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