A0A0W0DAZ4 · A0A0W0DAZ4_CANGB
- ProteinATP-dependent DNA helicase PIF1
- GenePIF1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids841 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, dna binding.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 280-287 | ATP (UniProtKB | ChEBI) | |||
DNA binding | 745-764 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial membrane | |
Cellular Component | nuclear replication fork | |
Cellular Component | site of double-strand break | |
Molecular Function | 5'-3' DNA helicase activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | centromeric DNA binding | |
Molecular Function | G-quadruplex DNA binding | |
Molecular Function | single-stranded DNA binding | |
Molecular Function | telomerase inhibitor activity | |
Molecular Function | telomeric DNA binding | |
Biological Process | DNA unwinding involved in DNA replication | |
Biological Process | double-strand break repair via break-induced replication | |
Biological Process | G-quadruplex DNA unwinding | |
Biological Process | mitochondrial genome maintenance | |
Biological Process | negative regulation of telomere maintenance via telomerase | |
Biological Process | replication fork reversal | |
Biological Process | telomere maintenance via recombination | |
Biological Process | translesion synthesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent DNA helicase PIF1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Nakaseomyces
Accessions
- Primary accessionA0A0W0DAZ4
Proteomes
Organism-specific databases
Subcellular Location
Interaction
Subunit
Monomer. Interacts with telomerase.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 272-569 | AAA+ ATPase | |||
Region | 799-841 | Disordered | |||
Sequence similarities
Belongs to the helicase family. PIF1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length841
- Mass (Da)95,238
- Last updated2016-03-16 v1
- Checksum0B79FC76DBF82337
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LLZZ01000155 EMBL· GenBank· DDBJ | KTA97920.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LLZZ01000122 EMBL· GenBank· DDBJ | KTB02965.1 EMBL· GenBank· DDBJ | Genomic DNA |