A0A0W0CLN7 · A0A0W0CLN7_CANGB
- ProteinDouble-strand break repair protein
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids656 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex 1 mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is 2 required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.
Cofactor
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 125 | Proton donor | ||||
Sequence: H |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDouble-strand break repair protein
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Nakaseomyces
Accessions
- Primary accessionA0A0W0CLN7
Proteomes
Organism-specific databases
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 291-473 | Mre11 DNA-binding | ||||
Sequence: PKLIPLKLDTVRTFKMKTIHLKDEPTLRPHDKEAISKYLVDIIEEMLDEANRETKEKLFPNIPMDEIESMGSFPLPLIRLRVDYSGPDENPEIIYQVENPRRFSNRFVGKIANTNNIVQFFRAKRKPIAKTRKDGVTTMDIDNELPFNDGSELQVDDLIADLLKKMNLSLLPENGMNEAMKKF | ||||||
Region | 550-656 | Disordered | ||||
Sequence: INKLPAKSSRNSRPSTRQKKNLSSEIIVSEDELESDHDADLKGYDEDLIEISSDEDNYRERRRVRSTKPTKSPKGSAKSSKRSATTRKSARTPKTDILGSLLAKKRK | ||||||
Compositional bias | 551-572 | Polar residues | ||||
Sequence: NKLPAKSSRNSRPSTRQKKNLS | ||||||
Compositional bias | 584-612 | Basic and acidic residues | ||||
Sequence: SDHDADLKGYDEDLIEISSDEDNYRERRR |
Sequence similarities
Belongs to the MRE11/RAD32 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length656
- Mass (Da)74,846
- Last updated2016-03-16 v1
- Checksum70A4539625C2C843
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 551-572 | Polar residues | ||||
Sequence: NKLPAKSSRNSRPSTRQKKNLS | ||||||
Compositional bias | 584-612 | Basic and acidic residues | ||||
Sequence: SDHDADLKGYDEDLIEISSDEDNYRERRR |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LLZZ01000139 EMBL· GenBank· DDBJ | KTB00318.1 EMBL· GenBank· DDBJ | Genomic DNA |