A0A0U5KFN5 · A0A0U5KFN5_CHIKV

Function

function

Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Catalytic activity

  • 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = L-aspartyl-[protein] + ADP-D-ribose + H+
    This reaction proceeds in the forward direction.
  • 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + ADP-D-ribose + H+
    This reaction proceeds in the forward direction.
  • ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.84 (UniProtKB | ENZYME | Rhea)
  • ATP + H2O = ADP + phosphate + H+
    EC:3.6.4.13 (UniProtKB | ENZYME | Rhea)
  • RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate
    EC:2.7.7.19 (UniProtKB | ENZYME | Rhea)
  • GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
  • N7-methyl-GTP + L-histidyl-[protein] = N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + diphosphate
    This reaction proceeds in the forward direction.
  • N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
  • a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H+
    This reaction proceeds in the forward direction.
    EC:3.6.1.74 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H+
    EC:3.6.1.15 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site1013For cysteine protease nsP2 activity
Active site1083For cysteine protease nsP2 activity

GO annotations

AspectTerm
Cellular Componentcytoplasmic vesicle membrane
Cellular Componenthost cell cytoplasmic vesicle membrane
Cellular Componenthost cell filopodium
Cellular Componenthost cell nucleus
Cellular Componenthost cell plasma membrane
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functioncysteine-type peptidase activity
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionmRNA methyltransferase activity
Molecular Functionpoly(A) RNA polymerase activity
Molecular Functionpolynucleotide 5'-phosphatase activity
Molecular Functionribonucleoside triphosphate phosphatase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Process7-methylguanosine mRNA capping
Biological ProcessDNA-templated transcription
Biological Processmethylation
Biological ProcessmRNA modification
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Polyprotein P1234
  • Alternative names
    • Non-structural polyprotein

Organism names

Accessions

  • Primary accession
    A0A0U5KFN5

Subcellular Location

Host cell membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Peripheral membrane protein
Host nucleus

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Interacts with RNA-directed RNA polymerase nsP4. Interacts with mRNA-capping enzyme nsP1. Interacts with KPNA1/karyopherin-alpha1; this interaction probably allows the active transport of protease nsP2 into the host nucleus.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain28-259Alphavirus-like MT
Region485-505Disordered
Domain690-991+RNA virus helicase C-terminal
Domain1004-1327Peptidase C9
Domain1324-1493Macro
Domain2224-2299RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    2,299
  • Mass (Da)
    256,523
  • Last updated
    2016-03-16 v1
  • Checksum
    AF6347ACE7164FFC
MDSVYVDIDADSAFLKALQRAYPMFEVEPRQVTSNDHANARAFSHLAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAAGKVLDRNISGKIGDLQEVMAVPDTETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLYHQAIKGVRVAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRRGKLSIMRGKKLKPCDRVLFSVGSTLYPESRMLLKSWHLPSVFHLKGKLSFTCRCDTVVSCEGYVVKRITMSPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICDQMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFSKWAKECRKDMEDEKLLGVRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVQAEFDSFVVPGLWSSGLSIPLRTRIKWLLRKVPKADLIPYSGNAQEAQDAEKEAEEEREAELTHEALPPLQAAQEDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQLTDHVVGEYLVLSPQTVLRSQKLSLIHALAEQVKTCTHSGRAGRYAVEAYDGRVLVPSGYAISPEDFQSLSESATMVYNEREFVNRKLHHIAMHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNPPYHEFAYEGLKIRPACPYKIAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEISTDVMRQRGLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRLKVVLCGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEYNMPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGHEVMTAAASQGLTRKGVYAVRQKVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEHASIMAGICSHQVTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDKAYSPEVALNEICTRMYGVDLDSGLFSKPLVSVYYADNHWDNRPGGKMFGFNPEAASILERKYPFTKGKWNINKQICVTTRRIEDFNPTTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKINGHHVLLVSGYNLALPTKRVTWVAPLGVRGADYTYNLELGLPATLGRYDLVVINIHTPFRIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVICVLGRKFRSSRALKPPCITSNTEMFFLFSSFDNGRRNFTTHVMNNQLNAAFVGQATRAGCAPSYRVKRMDIAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAVGPNFSNYTESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHLFTAMDSTDADVVIYCRDKEWEKKISEAIQMRTQVELLDEHISIDCDVIQVHPDSSLAGRKGYSTTEGALYSYLEGTRFHQTAVDVAEIHTMWPKQIEANEQVCLYALGESIESIRQKCPVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTNIIVCSSFPLPKYKIEGVQKVKCSKVMLFDHNVPSRVSPREYRSSQESVREVSMTTSLTHSQFDLSADGETLPVPSDLDADAPALEPALDDGAIHTTGNLAAVSDWVMSTVPVAPPRRRRGRNLTVICDEREGNITPMASVRFFRAEQCPTVQETAETRDTAISFRAPPSITVELSHPPISFGAPSETFPITFGDFNDGEIESLSSELLTFGDFLPGEVDDLTDSDWSTCSDTDDELXLDRAGGYIFSSDTGPGHLQQKSVRQSVLPVNTLEEVHEEKCYPPKLDELKEQLLLKKLQESASTANRSRYQSRKVENMKATIIQRLKRGCKLYLMAETPKVPTYRTVYPAPVYSPPINVRLSNPESAVAACNEFLARNYPTVSSYQITDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHAYHAPSIRSAVPSPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKKFACNREYWEEFAASPIRITTENLTTYVTKLKGPKAAALLARTHNLLPLQDVPMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGDAVLETDIASFDKSQDDSLALTALMLLEDLGVDHSLLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFVNT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LN898098
EMBL· GenBank· DDBJ
CUS27893.1
EMBL· GenBank· DDBJ
Genomic RNA

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