A0A0U5KFN5 · A0A0U5KFN5_CHIKV
- ProteinPolyprotein P1234
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids2299 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.
Catalytic activity
- 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = L-aspartyl-[protein] + ADP-D-ribose + H+This reaction proceeds in the forward direction.
- 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + ADP-D-ribose + H+This reaction proceeds in the forward direction.
- GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
- N7-methyl-GTP + L-histidyl-[protein] = N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + diphosphateThis reaction proceeds in the forward direction.
- N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 1013 | For cysteine protease nsP2 activity | ||||
Sequence: C | ||||||
Active site | 1083 | For cysteine protease nsP2 activity | ||||
Sequence: H |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended namePolyprotein P1234
- Alternative names
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Togaviridae > Alphavirus
- Virus hosts
Accessions
- Primary accessionA0A0U5KFN5
Subcellular Location
UniProt Annotation
GO Annotation
Host cell membrane ; Lipid-anchor
Host cytoplasmic vesicle membrane ; Lipid-anchor
Host cytoplasmic vesicle membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Interacts with RNA-directed RNA polymerase nsP4. Interacts with mRNA-capping enzyme nsP1. Interacts with KPNA1/karyopherin-alpha1; this interaction probably allows the active transport of protease nsP2 into the host nucleus.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 28-259 | Alphavirus-like MT | ||||
Sequence: EPRQVTSNDHANARAFSHLAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAAGKVLDRNISGKIGDLQEVMAVPDTETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLYHQAIKGVRVAYWVGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRRGKLSIMRGKKLKPCDRVLFSVGSTLYPESRMLLKSWH | ||||||
Region | 485-505 | Disordered | ||||
Sequence: GNAQEAQDAEKEAEEEREAEL | ||||||
Domain | 690-991 | +RNA virus helicase C-terminal | ||||
Sequence: DLTNPPYHEFAYEGLKIRPACPYKIAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEISTDVMRQRGLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRLKVVLCGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEYNMPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGHEVMTAAASQGLTRKGVYAVRQKVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEHAS | ||||||
Domain | 1004-1327 | Peptidase C9 | ||||
Sequence: DTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDKAYSPEVALNEICTRMYGVDLDSGLFSKPLVSVYYADNHWDNRPGGKMFGFNPEAASILERKYPFTKGKWNINKQICVTTRRIEDFNPTTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKINGHHVLLVSGYNLALPTKRVTWVAPLGVRGADYTYNLELGLPATLGRYDLVVINIHTPFRIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVICVLGRKFRSSRALKPPCITSNTEMFFLFSSFDNGRRNFTTHVMNNQLNAAFVGQ | ||||||
Domain | 1324-1493 | Macro | ||||
Sequence: FVGQATRAGCAPSYRVKRMDIAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAVGPNFSNYTESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHLFTAMDSTDADVVIYCRDKEWEKKISEAIQMRT | ||||||
Domain | 2224-2299 | RdRp catalytic | ||||
Sequence: DAVLETDIASFDKSQDDSLALTALMLLEDLGVDHSLLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFVNT |
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length2,299
- Mass (Da)256,523
- Last updated2016-03-16 v1
- ChecksumAF6347ACE7164FFC
Keywords
- Coding sequence diversity