A0A0U2GRF0 · A0A0U2GRF0_CVH22

  • Protein
    ORF1ab polyprotein
  • Gene
    orf1ab
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5687
Active site5689
Active site5788
Active site5864
Active site5869
Binding site5927-5933S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6350
Active site6365
Active site6406

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Gene names

    • Name
      orf1ab

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • Camel/Taif/T91
    • Camel/Taif/T96/2015
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus > Duvinacovirus > Human coronavirus 229E

Accessions

  • Primary accession
    A0A0U2GRF0

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1935-1958Helical
Transmembrane2005-2025Helical
Transmembrane2079-2096Helical
Transmembrane2103-2121Helical
Transmembrane2500-2522Helical
Transmembrane2735-2754Helical
Transmembrane2760-2777Helical
Transmembrane2784-2800Helical
Transmembrane2806-2824Helical
Transmembrane2831-2856Helical
Transmembrane3168-3189Helical
Transmembrane3283-3304Helical
Transmembrane3310-3329Helical
Transmembrane3334-3357Helical
Transmembrane3377-3395Helical
Transmembrane3402-3420Helical
Transmembrane3440-3464Helical
Transmembrane3471-3492Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond2028↔2055
Disulfide bond2046↔2052

Keywords

Interaction

Subunit

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain2-109CoV Nsp1 globular
Domain113-359CoV Nsp2 N-terminal
Domain389-775CoV Nsp2 middle
Domain773-897CoV Nsp2 C-terminal
Domain898-993Ubiquitin-like
Domain1023-1276Peptidase C16
Domain1275-1443Macro
Domain1607-1662Ubiquitin-like
Domain1670-1921Peptidase C16
Domain2012-20773Ecto
Region2151-2241Y1
Domain2151-2490CoV Nsp3 Y
Region2155-2168ZF1
Region2201-2211ZF2
Region2242-2490CoV-Y
Region2389-2490Y4
Domain2875-2970Nsp4C
Domain2971-3272Peptidase C30
Domain3552-3634RdRp Nsp7 cofactor
Domain3635-3829RdRp Nsp8 cofactor
Domain3830-3938Nsp9 ssRNA-binding
Domain3939-4077ExoN/MTase coactivator
Domain4079-4328NiRAN
Domain4334-4432Nsp12 Interface
Domain4433-5000Nsp12 RNA-dependent RNA polymerase
Domain4680-4842RdRp catalytic
Domain5001-5084CV ZBD
Domain5258-5612+RNA virus helicase C-terminal
Domain5669-5883ExoN
Domain5892-6113N7-MTase
Region6004-6018GpppA-binding
Domain6116-6176Nsp15 N-terminal oligomerization
Domain6177-6303AV-Nsp11N/CoV-Nsp15M
Domain6320-6460NendoU
Domain6464-6760Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,763
  • Mass (Da)
    753,321
  • Last updated
    2016-03-16 v1
  • Checksum
    C08A7E39B04FB786

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KT368913
EMBL· GenBank· DDBJ
ALA50234.1
EMBL· GenBank· DDBJ
Genomic RNA
KT368914
EMBL· GenBank· DDBJ
ALA50241.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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