A0A0U1UZ20 · A0A0U1UZ20_9ALPC

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5829
Active site5831
Active site5930
Active site6006
Active site6011
Binding site6069-6075S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6483
Active site6498
Active site6539

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BtMf-GD2012
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus > Minunacovirus > Miniopterus bat coronavirus HKU8

Accessions

  • Primary accession
    A0A0U1UZ20

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane2083-2101Helical
Transmembrane2141-2163Helical
Transmembrane2274-2295Helical
Transmembrane2641-2658Helical
Transmembrane2900-2921Helical
Transmembrane3419-3438Helical
Transmembrane3450-3469Helical
Transmembrane3474-3494Helical
Transmembrane3517-3535Helical
Transmembrane3580-3598Helical
Transmembrane3610-3632Helical

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain2-109CoV Nsp1 globular
Domain114-357CoV Nsp2 N-terminal
Domain891-987Ubiquitin-like
Domain1135-1387Peptidase C16
Domain1385-1551Macro
Domain1750-1805Ubiquitin-like
Domain1812-2069Peptidase C16
Domain2157-22213Ecto
Region2295-2385Y1
Domain2295-2634CoV Nsp3 Y
Region2299-2312ZF1
Region2345-2355ZF2
Region2386-2634CoV-Y
Region2533-2634Y4
Domain3015-3110Nsp4C
Domain3111-3412Peptidase C30
Domain3692-3774RdRp Nsp7 cofactor
Domain3775-3968RdRp Nsp8 cofactor
Domain3969-4078Nsp9 ssRNA-binding
Domain4079-4218ExoN/MTase coactivator
Domain4220-4469NiRAN
Domain4475-4573Nsp12 Interface
Domain4574-5141Nsp12 RNA-dependent RNA polymerase
Domain4821-4983RdRp catalytic
Domain5142-5225CV ZBD
Domain5389-5750+RNA virus helicase C-terminal
Domain5811-6025ExoN
Domain6034-6255N7-MTase
Region6146-6160GpppA-binding
Domain6258-6318Nsp15 N-terminal oligomerization
Domain6319-6436AV-Nsp11N/CoV-Nsp15M
Domain6453-6593NendoU
Domain6597-6893Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,896
  • Mass (Da)
    763,336
  • Last updated
    2016-03-16 v1
  • Checksum
    5648C083FA5D559A

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KJ473797
EMBL· GenBank· DDBJ
AIA62211.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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