A0A0U1RGY0 · SACA_NEIMA
- ProteinUDP-N-acetylglucosamine 2-epimerase
- GenesacA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids372 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the interconversion between UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-N-acetylmannosamine (UDP-ManNAc). Involved in the biosynthesis of the capsular polysaccharides. In vitro, can also use several chemoenzymatically synthesized UDP-ManNAc derivatives as substrates, with lower efficiency.
Catalytic activity
- UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
Activity regulation
Activated by UDP-GlcNAc and inhibited by 2-acetamidoglucal and UDP. Activity is strongly decreased in the presence of Co2+ and abolished in the presence of Mn2+ or Zn2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.6 mM | UDP-GlcNAc | |||||
5.2 mM | UDP-ManNAc | |||||
20 mM | UDP-ManNGc | |||||
51 mM | UDP-ManNPr | |||||
63 mM | rUDP-ManNAc |
kcat is 31 sec-1 with UDP-GlcNAc as substrate. kcat is 52 sec-1 with UDP-ManNAc as substrate. kcat is 4.2 sec-1 with UDP-ManNGc as substrate. kcat is 7.8 sec-1 with UDP-ManNPr as substrate. kcat is 6.7 sec-1 with rUDP-ManNAc as substrate.
pH Dependence
Optimum pH is 8-0-9.0.
Pathway
Capsule biogenesis; capsule polysaccharide biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10 | substrate | ||||
Sequence: R | ||||||
Binding site | 15 | substrate | ||||
Sequence: K | ||||||
Binding site | 95 | substrate | ||||
Sequence: D | ||||||
Binding site | 117 | substrate | ||||
Sequence: E | ||||||
Binding site | 212 | substrate | ||||
Sequence: H | ||||||
Binding site | 270 | substrate | ||||
Sequence: Q | ||||||
Binding site | 275 | substrate | ||||
Sequence: F | ||||||
Binding site | 289-291 | substrate | ||||
Sequence: SGG | ||||||
Binding site | 295 | substrate | ||||
Sequence: E | ||||||
Binding site | 312 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | UDP-N-acetylglucosamine 2-epimerase activity | |
Biological Process | capsule polysaccharide biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUDP-N-acetylglucosamine 2-epimerase
- EC number
- Short namesUDP-GlcNAc 2-epimerase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Neisseriales > Neisseriaceae > Neisseria
Accessions
- Primary accessionA0A0U1RGY0
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000442460 | 1-372 | UDP-N-acetylglucosamine 2-epimerase | |||
Sequence: MKVLTVFGTRPEAIKMAPVILELQKHNTITSKVCITAQHREMLDQVLSLFEIKADYDLNIMKPNQSLQEITTNIISSLTDVLEDFKPDCVLVHGDTTTTFAASLAAFYQKIPVGHIEAGLRTYNLYSPWPEEANRRLTSVLSQWHFAPTEDSKNNLLSESIPSDKVIVTGNTVIDALMVSLEKLKITTIKKQMEQAFPFIQDNSKVILITAHRRENHGEGIKNIGLSILELAKKYPTFSFVIPLHLNPNVRKPIQDLLSSVHNVHLIEPQEYLPFVYLMSKSHIILSDSGGIQEEAPSLGKPVLVLRDTTERPEAVAAGTVKLVGSETQNIIESFTQLIEYPEYYEKMANIENPYGIGNASKIIVETLLKNR |
Structure
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)41,731
- Last updated2016-02-17 v1
- ChecksumE3F9DDBEE6E8821A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL157959 EMBL· GenBank· DDBJ | CAM07513.1 EMBL· GenBank· DDBJ | Genomic DNA |