A0A0T9UI76 · A0A0T9UI76_YERAL

  • Protein
    tRNA sulfurtransferase
  • Gene
    thiI
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site184-185ATP (UniProtKB | ChEBI)
Binding site266ATP (UniProtKB | ChEBI)
Binding site288ATP (UniProtKB | ChEBI)
Binding site297ATP (UniProtKB | ChEBI)
Active site457Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA thio-modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      ERS137965_02995

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • IP06005
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Yersinia

Accessions

  • Primary accession
    A0A0T9UI76

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond345↔457Redox-active

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain62-166THUMP
Domain405-481Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    483
  • Mass (Da)
    54,791
  • Last updated
    2016-02-17 v1
  • Checksum
    E92C2E0DBA615A6B
MKFIIKLFPEITIKSQSVRLRFIKILTTNIRNVLKNLEDDTLAVVRHWDHIEIRTKDDNLGPQICDALTRVPGIHHILEVEDRSYTDMHNIFEQTLEAYRETLVGKTFCVRVKRRGKHEFSSGDVERYVGGGLNQHIESAKVKLTHPQVTVHLEVDQEKLTLIKARHEGLGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGSAHEIGVKQVAHYLWNRFGSSHRVRFVAIDFEPVVGEILEKVEDGQMGVVLKRMMVRAASQVAERYGVQALVTGEALGQVSSQTLTNLRLIDNASDTLILRPLISHDKEHIINLAREIGTEDFAKTMPEYCGVISKSPTVKAVKAKIEEEESHFDFSILERVVSEAKNVDIREIAEQSREQVVEVETVAELADSDVLLDIRAPDEQDEKPLKLEGVEVRALPFYKLSSQFADLDQSKTYLLYCDRGVMSRLQALYLREQGYTNVKVYRP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CQEJ01000018
EMBL· GenBank· DDBJ
CNL42084.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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