A0A0S9CH76 · A0A0S9CH76_9SPHN

  • Protein
    Multifunctional fusion protein
  • Gene
    cysN
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

APS kinase catalyzes the synthesis of activated sulfate.
Catalyzes the synthesis of activated sulfate.
Proposed to provide activated sulfate for transfer to Nod factor. ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.
With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site36-43GTP (UniProtKB | ChEBI)
Binding site115-119GTP (UniProtKB | ChEBI)
Binding site170-173GTP (UniProtKB | ChEBI)
Binding site473-480ATP (UniProtKB | ChEBI)
Active site547Phosphoserine intermediate

GO annotations

AspectTerm
Molecular Functionadenylylsulfate kinase activity
Molecular FunctionATP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionsulfate adenylyltransferase (ATP) activity
Biological Processhydrogen sulfide biosynthetic process
Biological Processsulfate assimilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Sulfate adenylyltransferase subunit 1
  • EC number
  • Alternative names
    • ATP-sulfurylase large subunit
    • Sulfate adenylate transferase
      (SAT
      )
  • Recommended name
    Adenylyl-sulfate kinase
  • EC number
  • Alternative names
    • APS kinase
    • ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
    • Adenosine-5'-phosphosulfate kinase

Gene names

    • Name
      cysN
    • Synonyms
      cysC
    • ORF names
      ASE95_10555

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Leaf231
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingomonas

Accessions

  • Primary accession
    A0A0S9CH76

Proteomes

PTM/Processing

Keywords

Interaction

Subunit

Heterodimer composed of CysD, the smaller subunit, and CysN.
Sulfate-activating enzymes, NodP and NodQ, may be physically associated.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain27-242Tr-type G
Region598-620Disordered
Compositional bias602-616Basic and acidic residues

Sequence similarities

Belongs to the APS kinase family.
In the C-terminal section; belongs to the APS kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    636
  • Mass (Da)
    70,148
  • Last updated
    2016-02-17 v1
  • Checksum
    34B993E015D6A7CA
MTDAPAYVVDELVARDIDAYLDSHRHKTMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALESDSRKVGTQGAEIDFALLVDGLAAEREQGITIDVAYRFFNTDKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSYLCHLIGIRNIVLAVNKMDLVGYDQATFDAIVADYAAFARSIGIETFTALPISGFRGDNITIRSAQTPWYDGPTLVEHLETVELLSSVDADKPFRLPVQWVNRPHLDFRGFAGLIASGIVRVGDAVRVLPSGKTSRVTRIVTLDGDVEQAVAGQSVTLCFADEIDCSRGDVIAAADRPPQAADQFEATLVWLADDALRVGRSYWLKLGTQTVSVTVQQPKYVVNVNTLEQLAAKTLDLNAIGVVELTTDRAIVFEPYADNRTLGGFILIDKLTNATVAAGMLHFSLRRAQNVHWQAIDIGRDAHATLKDQRPAVLWFTGLSGSGKSTIANLVEKKLHALGRHTFLLDGDNVRHGLNKDLGFTEADRIENIRRVGEVAKLMTDAGLIVLTAFISPFRAEREMVRTMLPGGEFVEVFVDTPFEEAERRDVKGLYKKARAGQLKNFTGIDSPYERPERPEVRIDTTATTPEDAAEEIVALLLRRS

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias602-616Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LMLS01000002
EMBL· GenBank· DDBJ
KQN93016.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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