A0A0S7YM78 · A0A0S7YM78_9BACT

Function

function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 divalent metal cations per subunit. Magnesium or manganese.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.
Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site29-30D-ribulose 5-phosphate (UniProtKB | ChEBI)
Binding site30Mg2+ 2 (UniProtKB | ChEBI)
Binding site30Mg2+ 1 (UniProtKB | ChEBI)
Binding site34D-ribulose 5-phosphate (UniProtKB | ChEBI)
Site128Essential for DHBP synthase activity
Binding site166D-ribulose 5-phosphate (UniProtKB | ChEBI)
Site166Essential for DHBP synthase activity
Binding site264-268GTP (UniProtKB | ChEBI)
Binding site269Zn2+ (UniProtKB | ChEBI); catalytic
Binding site280Zn2+ (UniProtKB | ChEBI); catalytic
Binding site282Zn2+ (UniProtKB | ChEBI); catalytic
Binding site285GTP (UniProtKB | ChEBI)
Binding site307-309GTP (UniProtKB | ChEBI)
Binding site330GTP (UniProtKB | ChEBI)
Active site342Proton acceptor; for GTP cyclohydrolase activity
Active site344Nucleophile; for GTP cyclohydrolase activity
Binding site365GTP (UniProtKB | ChEBI)
Binding site370GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3,4-dihydroxy-2-butanone-4-phosphate synthase activity
Molecular FunctionGTP binding
Molecular FunctionGTP cyclohydrolase II activity
Molecular Functionmagnesium ion binding
Molecular Functionmanganese ion binding
Molecular Functionzinc ion binding
Biological Processriboflavin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Riboflavin biosynthesis protein RibBA

Including 2 domains:

  • Recommended name
    3,4-dihydroxy-2-butanone 4-phosphate synthase
  • EC number
  • Short names
    DHBP synthase
  • Recommended name
    GTP cyclohydrolase-2
  • EC number
  • Alternative names
    • GTP cyclohydrolase II

Gene names

    • Name
      ribBA
    • ORF names
      AMS14_03335

Organism names

Accessions

  • Primary accession
    A0A0S7YM78

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-203DHBP synthase
Region204-415GTP cyclohydrolase II
Domain212-386GTP cyclohydrolase II

Sequence similarities

In the C-terminal section; belongs to the GTP cyclohydrolase II family.
In the N-terminal section; belongs to the DHBP synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    415
  • Mass (Da)
    45,086
  • Last updated
    2016-02-17 v1
  • Checksum
    1AB5C2A75E137D7D
MTGLFSDIPEILEDLRAGRTVVLVDDEQRENEGDLVCAAEKVTPEVINFMAAHGRGLVCLPMAPELIDRLNLPPQTADNTARFGTAFTVSVDARTGVTTGISAADRARTVQIAVADDTQPQDLARPGHVFPIRAREGGVLVRAGQTEGAVDLARAAGLKPAGVICEIMKADGTMARMPDLAAFCKEHNLKMCSVAQIIEFRHRKERLVRLVAQARLPTRHATFDLLAYESVVDAFPHIALTLGGIGIPGPDGTVPVQDEPVLVRIHSECLTGDVFGSLLCDCGQQLDTALARVAQDGRGAVIYMRQEGRGIGLTNKIRAYRLQQEEGLDTVEANERLGFQPDLREYGIGAQVMVELGIRQVRLMTNNPRKVVALEGYGLRLVERVPIIVPPNPMNARYLNTKRAKLGHLLDEDGS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LIZP01000054
EMBL· GenBank· DDBJ
KPJ75579.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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