A0A0S7BVG8 · A0A0S7BVG8_9CHLR

  • Protein
    DNA topoisomerase 1
  • Gene
    topA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

1802100200300400500600700800
TypeIDPosition(s)Description
Site137Interaction with DNA
Site243Interaction with DNA
Site244Interaction with DNA
Site247Interaction with DNA
Site252Interaction with DNA
Site259Interaction with DNA
Active site404O-(5'-phospho-DNA)-tyrosine intermediate
Site406Interaction with DNA
Site604Interaction with DNA

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      ATC1_13797

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • TC1
  • Taxonomic lineage
    Bacteria > Chloroflexota > Anaerolineae > Anaerolineales > Anaerolineaceae > Flexilinea

Accessions

  • Primary accession
    A0A0S7BVG8

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region51-100Disordered
Compositional bias81-98Polar residues
Domain107-217Toprim
Region267-272Interaction with DNA

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    802
  • Mass (Da)
    90,256
  • Last updated
    2016-02-17 v1
  • Checksum
    7947BE82021767FD
MEAYCLKCKQKREIEKPSAEYTSNGIPVTKGLCSVCGSKLSLLGYSTAHDGVPKPDSATASVPQKAISKDPKKRTAATRSGGKKSVKTQNTTTLKEEKPVKSGLSDQKLVIVESPAKAKTVGRFLGRGYAVRASIGHVRDLKKSTLSVSVENNFEPEYRVPNEKRQLVKELTELAKKSKKIYLATDPDREGEAIAWHLLESAAIDPERVDRVVFHEITQPAIKAAFDNPRSIDMNLVDAQQARRILDRLVGYKLSPLLWAKVQGHLSAGRVQSVAVRLIVDREREIENFKPKEYWTITVELQPEGVKGTFFSRLQKINGQDPELGDESIVQTHLENLKKAAFFIQSIKKGTRRRKPSAPFTTSTLQQEASKRLGFTANRTMALAQQLYEGVDIGSEGSTGLITYMRTDSTNISEIAQKDARAFIVNRFGSSFAPGKIPQYKTKSKGAQEAHEAIRPTDVNRTPDRMKEFLGRDQFRLYQLIWQRFLASQMEDAVYDTISIDVAANYQNQYLFRSSGSKLQFQGFLVLYEESQDEDFKFDEMENTKFPEGLFEGQRQKCLQYIPEQHFTQPPARYTEATLVKTLEENGIGRPSTYASILSTIQTRGYVSRESKRLFPTEIGIIVNDLLVKGFSSIVDVKFTSFLEEELDKVAEGEKDWRKVVGEFYSEFEPALNQANAELPKTKPEPEKVGRPCPECGRDLIIRQGRFGKFISCSGFPGCKYTEPIIQNIGIPCPICGKDIVVRRSKNGRIFYGCSAYPECQFVSWKKPVKTRCPECGGLMVEMNKNKIQCANCKHEIENPED

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias81-98Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DF968181
EMBL· GenBank· DDBJ
GAP40816.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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