A0A0S6U309 · A0A0S6U309_CLOBO

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site41(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site41dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site41isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site76(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site76dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site76isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site98[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site126(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site126dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site126isopentenyl diphosphate (UniProtKB | ChEBI)
Active site128Proton donor
Binding site162(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site190[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site218(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site218dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site218isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site219(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site219dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site219isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site220(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site220dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site220isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site262(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site262dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site262isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Molecular Functionnucleic acid binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      CBO05C_1979

Organism names

Accessions

  • Primary accession
    A0A0S6U309

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain303-372S1 motif
Domain390-458S1 motif
Domain479-547S1 motif
Domain564-633S1 motif

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    638
  • Mass (Da)
    71,814
  • Last updated
    2016-02-17 v1
  • Checksum
    53F3251805427D64
MNVILADKAGFCFGVKRALDTTINTKENLKGKIYTLGPLIHNNDVVNLLKGKGIEPINIGDVDKLNKDDTIIIRSHGVPLQTINYLKDKGLNVINTTCPHVANIQIKAKKYYEEGYKIIIVGDENHPEVIGINGWCNNSAIISKDGSNINNLDKKVCVLCQTTEKQENWEKVLNILIKKSREILAFNTICNATQVRQQAAKKLSEEVDSMVVIGGKNSSNTTKLYEICKNNCKNTIHVENAKEIPEHIYKNSNIIGVTAGASTPDWIIKEAVNKMNNSDNIIEISKNEMLEYMNEKEQQIVVGKVVKGTIVSLNENELFVDLNYKSEGIIPKEEVTLNEEAILKEAFKVGDEIEAKIVRIKNEDGYVVLSLKELDREKALKNLKETFENKETIKVIVKDAVDAGLICIYNGVRVFIPASHIELSHVDNLEKYKGSELEVNIIEFIKDRYKTKIVGSRREILKTIRDEHIEETWTSLEKDTIVEGEVKRFTNFGAFVEINGVDGLLHVSEISWGRVEKPEDALKIGDKIKVYILDIDKENKKLALSIKKLIEDPWKSVEIKYPIGNIVLGKVVRFASFGAFIELEPGVDGLVHISKISNKRIDKPEEELTLGKEVKAKILEVNSAEKRIALSIKDVEEF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DF384213
EMBL· GenBank· DDBJ
GAE02289.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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