A0A0S6TZ81 · A0A0S6TZ81_CLOBO
- ProteinATP-dependent helicase/nuclease subunit A
- GeneaddA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1279 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | DNA helicase complex | |
Molecular Function | 3'-5' DNA helicase activity | |
Molecular Function | 3'-5' exonuclease activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | double-stranded DNA binding | |
Molecular Function | isomerase activity | |
Biological Process | double-strand break repair via homologous recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent helicase/nuclease subunit A
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionA0A0S6TZ81
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Heterodimer of AddA and AddB/RexB.
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-499 | UvrD-like helicase ATP-binding | ||||
Sequence: TKWTDEQRQAIFTKNCNLLVAAGAGAGKTAVLVQRIIEKILDKEEPIDIDKLLVVTFTNAAAAEMRERIGDAISKGLDEEPESKVLRKQLTLLNKSNIMTIHSFCLQVIKNNFHTIEIDPNFRICDETEGILMKQEAMDELFDELYEIENEDFINLVESYASRKDTRLQEVVLELHRFAKSAPLPYDWLLNMAEGFNVGEEFNFEETLWADMIMEDMKVLLHGFKNMLQQSIEVILNSEGIDYYYEPFKMDLNFINNLLQKSSFKEFRNEIIVYDFPKLPVKRNKDADKEVKERVKKLRDKVKKKIVELKNILDSYENEFIRKEFIFLYPSMKALSNLVILFDKKYEAKKRERDLIDFNDIEHLCLSILTDKTDEGHIIPSNIALDYRKKFAEVLIDEYQDSNLVQEVIMSMVSRVKGYWSFSNSQLVFNEEEINFEEPQVGLDIPNRFMVGDVKQSIYRFRQAKPEIFLDKYNQYSEEEGSKYRKIKLFKNFRSR | ||||||
Coiled coil | 295-322 | |||||
Sequence: KERVKKLRDKVKKKIVELKNILDSYENE | ||||||
Domain | 526-853 | UvrD-like helicase C-terminal | ||||
Sequence: KEALTVGASYGEEVKGEPIELCLMDKKYEISEEVLKEYNMDEEEALDNIQLEGRLVAKKIQEVIGNNLEGGLKVFDKKLGEYRRIQYRDIVILMRATSNWAPVFVEELAKEGIPVFADTNSGYFDTTEIKTIISLLQIIDNPLQDIPLLSVLRSPIASFTDDDLIDIRMVNKNIAFYECMEIIYRLYKNERLDSYYSFYIEDEDKINRIVKDMKEELKNKICSFIEKLNLWRKKSIHIDIDEFIWFLYVETGYYGYVGALPAGEQRQANLRILFQRAKQYEKTSYKGLFNFINFINKLKFSSGDMGSAKILGENENVVRIMSIHKSKG |
Sequence similarities
Belongs to the helicase family. AddA subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,279
- Mass (Da)150,509
- Last updated2016-02-17 v1
- Checksum274E035CE1A76C14