A0A0S3RJD9 · A0A0S3RJD9_PHAAN
- ProteinAdenylyltransferase and sulfurtransferase MOCS3
- GeneVigan.03G027900
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids457 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Catalytic activity
- [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H+ = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate
Cofactor
Note: Binds 1 zinc ion per subunit.
Pathway
Cofactor biosynthesis; molybdopterin biosynthesis.
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 94 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 115 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 122-126 | ATP (UniProtKB | ChEBI) | ||||
Sequence: NNMHR | ||||||
Binding site | 139 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 183-184 | ATP (UniProtKB | ChEBI) | ||||
Sequence: DN | ||||||
Binding site | 224 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 227 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Active site | 241 | Glycyl thioester intermediate; for adenylyltransferase activity | ||||
Sequence: C | ||||||
Binding site | 299 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 302 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Active site | 415 | Cysteine persulfide intermediate; for sulfurtransferase activity | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | molybdopterin-synthase adenylyltransferase activity | |
Molecular Function | molybdopterin-synthase sulfurtransferase activity | |
Molecular Function | thiosulfate sulfurtransferase activity | |
Molecular Function | URM1 activating enzyme activity | |
Biological Process | Mo-molybdopterin cofactor biosynthetic process | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenylyltransferase and sulfurtransferase MOCS3
- Alternative names
Including 2 domains:
- Recommended nameMolybdopterin-synthase adenylyltransferase
- EC number
- Alternative names
- Recommended nameMolybdopterin-synthase sulfurtransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Vigna
Accessions
- Primary accessionA0A0S3RJD9
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for coiled coil, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 3-37 | |||||
Sequence: ASEILRELDSLKDAKAKIEHKISALEAQLREINLR | ||||||
Domain | 353-455 | Rhodanese | ||||
Sequence: KKEPHVLVDVRPAHHFKIVSLPKSLNIPLSTLESRLPEISAALKKEEDSGLVSGSGAQLYVVCRRGNDSQRAVQCLHKMGFTSAKDIVGGLESWAYNVDPKFP |
Sequence similarities
In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length457
- Mass (Da)49,486
- Last updated2016-02-17 v1
- ChecksumE522B0F0156E3C11
Keywords
- Technical term