A0A0S3RDL4 · A0A0S3RDL4_PHAAN
- ProteinQueuine tRNA-ribosyltransferase catalytic subunit 1
- GeneVigan.02G117900
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids461 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.
Catalytic activity
- guanosine34 in tRNA + queuine = guanine + queuosine34 in tRNA
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 140 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 140-144 | substrate | ||||
Sequence: DSGGF | ||||||
Binding site | 194 | substrate | ||||
Sequence: D | ||||||
Binding site | 237 | substrate | ||||
Sequence: Q | ||||||
Binding site | 264 | substrate | ||||
Sequence: G | ||||||
Active site | 314 | Nucleophile | ||||
Sequence: D | ||||||
Binding site | 352 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 354 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 357 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 382 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA-guanosine(34) queuine transglycosylase activity | |
Biological Process | tRNA-guanine transglycosylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameQueuine tRNA-ribosyltransferase catalytic subunit 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Vigna
Accessions
- Primary accessionA0A0S3RDL4
Proteomes
Subcellular Location
Interaction
Subunit
Heterodimer of a catalytic subunit and an accessory subunit.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 62-414 | tRNA-guanine15 transglycosylase-like | ||||
Sequence: RARAARLTLPHHVCQTPLFMPVGTQGTIKGLTNSQLEDIGCQIILGNTYHLALRPTSELLDEVGGLHNFMNWQRGLLTDSGGFQMVSLLHLADITEKGVTFQSPVDGKPMLLTPEESIQIQNRIGADIIMALDDVVKTTITGPRVEEAMYRTLRWIDRCIAAHKRPHDQNLFGIVQGGLDPILRDICAKGLVERNLPGYAIGGLSGGEDKNSFWRVVAQCTAALPEDKPRYVMGVGYPLDIVVCSALGADMYDCVYPTRTARFGTALIPEGVLKLKHKAMAEDTRPIDPTCPCMVCKNYTRAYIHCLVTKDAMGSQLLSYHNLYYMLQLSRNLHFSIVEGRFPEFVCDFLHKM | ||||||
Region | 295-301 | RNA binding | ||||
Sequence: GVGYPLD | ||||||
Region | 319-323 | RNA binding; important for wobble base 34 recognition | ||||
Sequence: TRTAR |
Sequence similarities
Belongs to the queuine tRNA-ribosyltransferase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length461
- Mass (Da)51,210
- Last updated2016-02-17 v1
- ChecksumD20D6A89DC786A27
Keywords
- Technical term