A0A0S3RDL4 · A0A0S3RDL4_PHAAN

  • Protein
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • Gene
    Vigan.02G117900
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site140Proton acceptor
Binding site140-144substrate
Binding site194substrate
Binding site237substrate
Binding site264substrate
Active site314Nucleophile
Binding site352Zn2+ (UniProtKB | ChEBI)
Binding site354Zn2+ (UniProtKB | ChEBI)
Binding site357Zn2+ (UniProtKB | ChEBI)
Binding site382Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase catalytic subunit 1
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      Vigan.02G117900
    • ORF names
      VIGAN_02117900

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Shumari
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Vigna

Accessions

  • Primary accession
    A0A0S3RDL4

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterodimer of a catalytic subunit and an accessory subunit.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain62-414tRNA-guanine15 transglycosylase-like
Region295-301RNA binding
Region319-323RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    461
  • Mass (Da)
    51,210
  • Last updated
    2016-02-17 v1
  • Checksum
    D20D6A89DC786A27
MFRGMSLQAPITWCLCFKPKPKTNTTNMLPPLHSRTRSRQGYTTTRTKAMALVFEVLGRFNRARAARLTLPHHVCQTPLFMPVGTQGTIKGLTNSQLEDIGCQIILGNTYHLALRPTSELLDEVGGLHNFMNWQRGLLTDSGGFQMVSLLHLADITEKGVTFQSPVDGKPMLLTPEESIQIQNRIGADIIMALDDVVKTTITGPRVEEAMYRTLRWIDRCIAAHKRPHDQNLFGIVQGGLDPILRDICAKGLVERNLPGYAIGGLSGGEDKNSFWRVVAQCTAALPEDKPRYVMGVGYPLDIVVCSALGADMYDCVYPTRTARFGTALIPEGVLKLKHKAMAEDTRPIDPTCPCMVCKNYTRAYIHCLVTKDAMGSQLLSYHNLYYMLQLSRNLHFSIVEGRFPEFVCDFLHKMFPKGDVPEWVCNAMEVAGIDISSCCAPFSSCEESVSKSMPEEVILAG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP015035
EMBL· GenBank· DDBJ
BAT78495.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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