A0A0S3RAU5 · A0A0S3RAU5_PHAAN
- ProteinBifunctional dihydrofolate reductase-thymidylate synthase
- GeneVigan.02G008400
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids529 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP
Pathway
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 411 | |||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Molecular Function | dihydrofolate reductase activity | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | methylation | |
Biological Process | one-carbon metabolic process | |
Biological Process | tetrahydrofolate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional dihydrofolate reductase-thymidylate synthase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Vigna
Accessions
- Primary accessionA0A0S3RAU5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-22 | Disordered | ||||
Sequence: MASDFSVISNGDSNGNVNPQPN | ||||||
Domain | 25-202 | DHFR | ||||
Sequence: TYQVVVAATQDWGIGKDGKLPWRLPTDLKFFKEITEKTSDPGKKNAIVMGRKTWESIPLQYRPLSGRLNVVLTRSGSFDIATAENVVICGSINSALELLASSPYSLSIEKVFVIGGGQIFREALNAPGCEAIHLTEIQSNIECDTFMPSVDFTIFRPWYSSFPKMENNIRHSFISYVR |
Sequence similarities
In the C-terminal section; belongs to the thymidylate synthase family.
In the N-terminal section; belongs to the dihydrofolate reductase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length529
- Mass (Da)59,855
- Last updated2016-02-17 v1
- ChecksumB7172DDEFBB9E12D
Keywords
- Technical term