A0A0R7VJL2 · A0A0R7VJL2_9REOV

  • Protein
    RNA-directed RNA polymerase
  • Gene
    VP1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    2/5

Function

function

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral +RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsRNA formation. To do so, the polymerase specifically recognizes and binds 4 bases 5'-UGUG-3' in the conserved 3'-sequence of plus-strand RNA templates. VP2 presumably activates the autoinhibited VP1-RNA complex to coordinate packaging and genome replication. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

GO annotations

AspectTerm
Cellular Componentvirion component
Molecular Functionnucleotide binding
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Biological ProcessDNA-templated transcription
Biological Processviral genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-directed RNA polymerase
  • EC number
  • Alternative names
    • Protein VP1

Gene names

    • Name
      VP1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • RVA/Human-wt/PHI/TGO12-016/2012/G1P[8]
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Duplornaviricota > Resentoviricetes > Reovirales > Sedoreoviridae > Rotavirus > Rotavirus A

Accessions

  • Primary accession
    A0A0R7VJL2

Proteomes

Subcellular Location

Virion
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Keywords

  • Cellular component

Interaction

Subunit

Interacts with VP3 (Potential). Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain501-687RdRp catalytic

Sequence similarities

Belongs to the reoviridae RNA-directed RNA polymerase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,088
  • Mass (Da)
    125,095
  • Last updated
    2016-02-17 v1
  • Checksum
    8D6B574D2DAD4AA0
MGKYNLILSEYLSFIYNSQSAVQIPIYYSSNSELESRCIEFHSKCLENSKNGLSLRKLFNEYSDVIENATLLSILSYSYDKYNAVERKLVKYAKGKPLEADLTVNELDYENNKITSELFPTAEEYTDSLMDPAILTSLSSNLNAVMFWLEKHENDTAEKFKIYKRRLDLFTIVASTVNKYGVPRHNAKYRYEYDVMKDKPYYLVTWANSSIEMLMSVFSHEDYLIARELIVLSYSNRSTLAKLVSSPMSILVALVDINGTFITNEELELEFSNKYVRAIVPDQTFDELKQMLDNMRKAGLVDIPKMIQDWLVDCSIEKFPLMAKIYSWSFHVGFRKQKMLDAALDQLKTEYTEDVDDEMYREYTMLIRDEVVKMLEESVKHDDHLLQDSELAGLLSMSSASNGESRQLKFGRKTVFSTKKNMHVMDDMANGKYTPGIIPPVNADKPIPLGRRDVPGRRTRIIFILPYEYFIAQHAVVEKMLIYAKHTREYAEFYSQSNQLLSYGDVTRFLSNNAMVLYTDVSQWDSSQHNTQPFRKGIIMGLDILANMTNDVKVIQTLNLYKQTQINLMDSYVQIPDGNVIKKIQYGAVASGEKQTKAANSIANLALIKTVLSRISNKYSFATKIIRVDGDDNYAVLQFNTEVTKQMVQDVSNDVRETYARMNAKVKALVSTVGIEIAKRYIAGGKIFFRAGINLLNNEKRGQSTQWDQAAVLYSNYIVNRLRGFETDREFILTKIMQMTSVAITGSLRLFPSERVLTTNSTFKVFDSEDFIIEYGTTDDEVYIQRAFMSLSSQRSGIADEIAASPTFKNYVSKLSEQLLFSKNNIVSRGIALTEKAKLNSYAPISLEKRRAQISALLTMLQKPVTFKSNKITINDILKDIKPFFTVNEAHLPIQYQKFMPTLPENVQYIVQCIGSRTYQIEDDGSKSAIFRLISKYSVYKPSIEELYKVISLHENEIQLYLISLGIPKIDADTYVGSKIYSQDKYRILESYVYNLLSINYGCYQLFDFNSPDLEKLIRIPFKGKIPAVTFILHLYAKLEVINHAIKNGSWISLFCNYPKSEMIKLWKKMWNITSLRSPYTNANFFQD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KP007193
EMBL· GenBank· DDBJ
AKS44548.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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