A0A0R2HMF5 · A0A0R2HMF5_9FIRM

Function

function

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site52-56(6S)-NADPHX (UniProtKB | ChEBI)
Binding site53K+ (UniProtKB | ChEBI)
Binding site120K+ (UniProtKB | ChEBI)
Binding site124-130(6S)-NADPHX (UniProtKB | ChEBI)
Binding site135(6S)-NADPHX (UniProtKB | ChEBI)
Binding site154(6S)-NADPHX (UniProtKB | ChEBI)
Binding site157K+ (UniProtKB | ChEBI)
Binding site254(6S)-NADPHX (UniProtKB | ChEBI)
Binding site314(6S)-NADPHX (UniProtKB | ChEBI)
Binding site365(6S)-NADPHX (UniProtKB | ChEBI)
Binding site399-403AMP (UniProtKB | ChEBI)
Binding site427AMP (UniProtKB | ChEBI)
Binding site428(6S)-NADPHX (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionADP-dependent NAD(P)H-hydrate dehydratase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular FunctionNADHX epimerase activity
Molecular FunctionNADPHX epimerase activity
Biological Processmetabolite repair
Biological Processnicotinamide nucleotide metabolic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional NAD(P)H-hydrate repair enzyme
  • Alternative names
    • Nicotinamide nucleotide repair protein

Including 2 domains:

  • Recommended name
    ADP-dependent (S)-NAD(P)H-hydrate dehydratase
  • EC number
  • Alternative names
    • ADP-dependent NAD(P)HX dehydratase
  • Recommended name
    NAD(P)H-hydrate epimerase
  • EC number

Gene names

    • Name
      nnrE
    • Synonyms
      nnrD
    • ORF names
      IV49_GL001104

Organism names

  • Taxonomic identifier
  • Strain
    • DSM 20405
  • Taxonomic lineage
    Bacteria > Bacillota > Erysipelotrichia > Erysipelotrichales > Coprobacillaceae > Kandleria

Accessions

  • Primary accession
    A0A0R2HMF5

Proteomes

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain9-211YjeF N-terminal
Domain219-485YjeF C-terminal

Sequence similarities

Belongs to the NnrD/CARKD family.
Belongs to the NnrE/AIBP family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    491
  • Mass (Da)
    53,945
  • Last updated
    2016-01-20 v1
  • Checksum
    6DF665794014E1F4
MYVGSQNQLKAYDAYLIEHGYTIEQLIDYASDALLKHISSYQHIGILVGPGNNGADGLSLGLKLDETGKDVCIYYIGDYSRFSQGNRYYFNQCLDQLRMINLDETFIDEKELNRHDVIVDAFFGFGLNSAPRGLYKSVIDLINLSYRGDVIAVDCPTGLNCNTGTPYSTVLFAQKTIALSALKEGFLNPESQVFTGDVVVEELAIDNPFMEAGLYALFDKQDAQSMIKQRRYDGYKNMYGIDLLICGSHQYRGAPILSAKGALYSGAGIIKVMSDEKVIDLLPLSLPECIGISRYKPITKEELQGYDAILIGCGLSLEKESYLLTSDVLVNANSPLVIDADALTILSTNMSLLKDISVPVILTPHIGEFKRMWPYEEGADLMEEATAFAKAYDVILVLKGPHTIITDGTEHYRIASGNKAMAVGGMGDTLAGMITSFLGQGYAPLSAALLGTYIHGFTGDILARKRYTVMPELLAEQIPCAMYILSQKIEE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JQBL01000003
EMBL· GenBank· DDBJ
KRN51031.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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