A0A0Q4E1L2 · A0A0Q4E1L2_9SPHN

  • Protein
    Glutamine-dependent NAD(+) synthetase
  • Gene
    nadE
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site54Proton acceptor; for glutaminase activity
Active site123For glutaminase activity
Binding site129L-glutamine (UniProtKB | ChEBI)
Active site179Nucleophile; for glutaminase activity
Binding site206L-glutamine (UniProtKB | ChEBI)
Binding site212L-glutamine (UniProtKB | ChEBI)
Binding site369-376ATP (UniProtKB | ChEBI)
Binding site459deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site483ATP (UniProtKB | ChEBI)
Binding site488deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site493-496deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site639deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamine-dependent NAD(+) synthetase
  • EC number
  • Alternative names
    • NAD(+) synthase [glutamine-hydrolyzing]

Gene names

    • Name
      nadE
    • ORF names
      ASE69_16225

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Leaf208
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingomonas

Accessions

  • Primary accession
    A0A0Q4E1L2

Proteomes

Subcellular Location

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain14-279CN hydrolase
Region644-663Disordered

Sequence similarities

In the C-terminal section; belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    681
  • Mass (Da)
    73,672
  • Last updated
    2016-01-20 v1
  • Checksum
    72B45F361BA0999D
MTHPFYAIHSHGLIRVAAATPRASVGDTGANAEATIALAREADAKGVDLVVFPELNLTSYAIDDLHLQTAQQRATLAAIGTVVEASAILRPVLLVGAALVRNGRLYNCALAIARGHVLGVVPKTFLPNYREFYEKRWFASGMGMTGLTIDLDGEAIPFGTDLIFAARDLPGFVFHAEICEDYWAPTPPSTAGALAGALICCNLSASNIVIGKARDRAMLAAAQSARAVCAYVYSAAGPGESTTEVAWDGQGLVHELGEQIAESARFSTEPELVVADVDCERITQERLRTGTFNDAAMFAGNPETRFRRIAFDHAPVLEDAGLERAIRRFPFVPNDPARRGDDCYEAFNIQVEALSKRLVSANAETIVIGVSGGLDSTHALIVAAKAMDRLGRPRSAILGFTMPGFATGEGTKANAWALMRALGITAEEIDIRPAATRMLEDIGHPFSEGEPVYDITFENVQAGLRTDYLFRFANQRRGIVLGTGDLSEMALGWSTYGVGDQMSHYAVNSGVPKTLIQFLIRWCIRTDQYDAATDAILSTILAQEISPELVPAGADGALQSTESMIGPYALNDFFAHYVIRYGLAPSKIAFLAWHAWRDASVGAWPEDLPADARVAYDLPTIRHWLEKFLFRFFTTSQFKRSAMPNGPKVSAGGALSPRGDWRAPSDGTAEIWIKELRSSVP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LMKH01000037
EMBL· GenBank· DDBJ
KQM46707.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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