A0A0P0WC75 · A0A0P0WC75_ORYSJ
- ProteinDol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids497 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Dol-P-Glc:Glc2Man9GlcNAc2-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc2Man9GlcNAc2-PP-Dol to produce Glc3Man9GlcNAc2-PP-Dol.
Catalytic activity
- a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + an alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol = a alpha-D-Glc-(1->2)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H+This reaction proceeds in the forward direction.
a di-trans,poly-cis-dolichyl β-D-glucosyl phosphate RHEA-COMP:19502 + an α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19522 = a α-D-Glc-(1→2)-α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19512 + a di-trans,poly-cis-dolichyl phosphate RHEA-COMP:19498 + CHEBI:15378
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity | |
Biological Process | dolichol-linked oligosaccharide biosynthetic process | |
Biological Process | leaf development | |
Biological Process | response to salt stress |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionA0A0P0WC75
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 50-69 | Helical | ||||
Sequence: GLYYISLAYVASLFPGAWVT | ||||||
Transmembrane | 89-106 | Helical | ||||
Sequence: VIMAMICGVLVHDLLLCI | ||||||
Transmembrane | 118-136 | Helical | ||||
Sequence: FAILVALYPIHWFFTFLYY | ||||||
Transmembrane | 142-170 | Helical | ||||
Sequence: LAAVLAMYLFCLKKQFWVSAAFGAFSILL | ||||||
Transmembrane | 269-289 | Helical | ||||
Sequence: VLIAFIPFAMVLLVFVAFIVW | ||||||
Transmembrane | 309-326 | Helical | ||||
Sequence: QFLYFGLVSAAALLPWHF | ||||||
Transmembrane | 347-370 | Helical | ||||
Sequence: FAILVALGLSLVAVHLFSIAHPYL | ||||||
Transmembrane | 390-411 | Helical | ||||
Sequence: WMMKYILTPVYVYSWFSIVNIL | ||||||
Transmembrane | 418-441 | Helical | ||||
Sequence: LWVLSFVLSVALALIPAPLVEFRY | ||||||
Transmembrane | 461-481 | Helical | ||||
Sequence: LLALGFLYAAVDFFTLAMFLF |
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 20-39 | Disordered | ||||
Sequence: GEEPVRVGDGEADRRRGSGR | ||||||
Compositional bias | 23-39 | Basic and acidic residues | ||||
Sequence: PVRVGDGEADRRRGSGR | ||||||
Region | 211-231 | Disordered | ||||
Sequence: NKEASHMDNKTTAPGLRRRRN |
Sequence similarities
Belongs to the ALG10 glucosyltransferase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length497
- Mass (Da)56,520
- Last updated2020-02-26 v1
- ChecksumC76A29E8A9ACAD09
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: A | ||||||
Compositional bias | 23-39 | Basic and acidic residues | ||||
Sequence: PVRVGDGEADRRRGSGR |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP008210 EMBL· GenBank· DDBJ | BAF15156.1 EMBL· GenBank· DDBJ | Genomic DNA |