A0A0P0VIP0 · LRSK7_ORYSJ

Function

function

Legume-lectin receptor-like kinase required for normal pollen development and male fertility (PubMed:31833176, PubMed:32284546).
Regulates pollen exine assembly and aperture development (PubMed:31833176, PubMed:32284546).
Plays a critical role in annulus formation, and may participate in the formation of the fibrillar-granular layer underneath the operculum (PubMed:32284546).
May function by regulating the expression of genes involved in pollen exine development (PubMed:31833176).
Kinase activity is required for its function in pollen development (PubMed:31833176).

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site395-403ATP (UniProtKB | ChEBI)
Binding site418ATP (UniProtKB | ChEBI)
Active site514Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Cellular Componentpollen aperture
Molecular FunctionATP binding
Molecular Functioncarbohydrate binding
Molecular Functionkinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processdefense response to bacterium
Biological Processdefense response to oomycetes
Biological Processpollen aperture formation
Biological Processpollen development
Biological Processprotein autophosphorylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    L-type lectin-domain containing receptor kinase S.7
  • EC number
  • Short names
    OsLecRK-S.7
  • Alternative names
    • Protein DEFECTIVE IN APERTURE FORMATION 1
      (OsDAF1
      )

Gene names

    • Name
      LECRKS7
    • Synonyms
      DAF1
    • Ordered locus names
      Os02g0459600
      , LOC_Os02g26160

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    A0A0P0VIP0
  • Secondary accessions
    • Q0E1D9
    • Q6K3K2

Proteomes

Genome annotation databases

Subcellular Location

Cell membrane
; Single-pass type I membrane protein
Cytoplasm, cytosol
Note: During meiosis, localizes diffusely in the cytosol and plasma membrane of microspore mother cells (MMCs). During tetrad development, localizes at the corners. At late tetrad stage, accumulates to the four corners of the tetrad, assembled into ring-like structures marking future aperture sites. When microspores are released from tetrads and preliminary aperture structures has formed, remains in a distinctly ring-shaped distribution beneath the aperture in the plasma membrane between the annulus and operculum.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain24-331Extracellular
Transmembrane332-352Helical
Topological domain353-695Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Male sterility due to aborted pollen grains with abnormal exine an intine, and defective aperture (PubMed:32284546).
Aborted pollen grains with defective apertures and intine formation, and male sterility (PubMed:32284546).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis418Loss of kinase activity.
Mutagenesis560In s13283; loss of kinase activity and male sterile phenotype.

PTM/Processing

Features

Showing features for signal, chain, glycosylation, modified residue.

TypeIDPosition(s)Description
Signal1-23
ChainPRO_500605635024-695L-type lectin-domain containing receptor kinase S.7
Glycosylation45N-linked (GlcNAc...) asparagine
Glycosylation279N-linked (GlcNAc...) asparagine
Modified residue376Phosphothreonine
Modified residue378Phosphoserine
Modified residue386Phosphothreonine
Modified residue403Phosphothreonine
Modified residue657Phosphothreonine

Post-translational modification

Autophosphorylated at Thr-376; Ser-378; Thr-386; Thr-403 and Thr-657.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in roots, leaves, lemma, palea, pistil and anthers.

Interaction

Subunit

Interacts with INP1 (PubMed:32284546).
Interaction with INP1 is required for DAF1 polar localization at the future aperture sites in tetrads (PubMed:32284546).

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region37-276Legume-lectin like
Compositional bias286-319Pro residues
Region286-323Disordered
Domain389-661Protein kinase

Sequence similarities

In the N-terminal section; belongs to the leguminous lectin family.
In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    695
  • Mass (Da)
    74,135
  • Last updated
    2016-01-20 v1
  • Checksum
    2F534B41133DB0C5
MPPRCRRLPLLFILLLAVRPLSAAAASSIAAAPASSYRRISWASNLTLLGSASLLPGAAGVALTTPSRDGVGAGRALFSEPVRLLLPQDAAASASASRAATPASFSTRFTFRITPSPTYGDGLAFLLTSSRTFLGASNGFLGLFPSSSASDEGELRDVSTVAVEIDTHLDVALHDPDGNHVALDAGSIFSVASAQPGVDLKAGVPITAWVEYRAPRRRLNVWLSYSPSRRPEKPALSADVDLSGLLRTYMYAGFSASNGNGAALHVVERWTFRTFGFPNSSYAPPPTKYIGPMPPNNQPLPPPPSPSPSPPPPSPPPPPHPNHRRRHLFYKVLGGVLGGMVLLGLVVVGSAVLLGRSVRRKNQEHAVASEDMGEATLSMEVARAATKGFDSGNVIGVGGSGATVYEGVLPSGSRVAVKRFQAIGSCTKAFDSELKAMLNCPHHPNLVPLAGWCRSKDELVLVYEFMPNGNLDSALHTLGGATLPWEARFRAVYGVASALAYLHDECENRIIHRDVKSSNVMLDAEFNARLGDFGLARTVSHGGLPLTTQPAGTLGYLAPEYVHTGVATERSDVYSFGVLALEVATGRRPAERGISVVNWVWTLWGRRRLVDAADRRLQGRFVADEMRRVLLVGLCCVHPDCRKRPGMRRVVSMLDGTAPLILVPDKMPPVLLQPVPNASSMNSADTANTAFFSCR

Sequence caution

The sequence BAD19984.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence BAF08699.2 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias286-319Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP005650
EMBL· GenBank· DDBJ
BAD19984.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AP008208
EMBL· GenBank· DDBJ
BAF08699.2
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
AP014958
EMBL· GenBank· DDBJ
BAS78542.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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