A0A0P0E4M9 · A0A0P0E4M9_HPV51
- ProteinReplication protein E1
- GeneE1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids634 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | hydrolase activity, acting on acid anhydrides | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplication protein E1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cossaviricota > Papovaviricetes > Zurhausenvirales > Papillomaviridae > Firstpapillomavirinae > Alphapapillomavirus > Alphapapillomavirus 5
- Virus hosts
Accessions
- Primary accessionA0A0P0E4M9
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 91 | Phosphoserine; by host | ||||
Sequence: S | ||||||
Modified residue | 95 | Phosphoserine; by host | ||||
Sequence: S |
Post-translational modification
Phosphorylated.
Keywords
- PTM
Expression
Keywords
- Developmental stage
Interaction
Subunit
Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.
Family & Domains
Features
Showing features for motif, region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 85-87 | Nuclear localization signal | ||||
Sequence: KRK | ||||||
Region | 92-117 | Disordered | ||||
Sequence: PRSSPLGDITNQNNTHSHSQANESQV | ||||||
Compositional bias | 95-117 | Polar residues | ||||
Sequence: SPLGDITNQNNTHSHSQANESQV | ||||||
Region | 172-338 | DNA-binding region | ||||
Sequence: CANVELNSICEVLKSSNAKATLMAKFKELYGISYNELVRVFKSDKTCCIDWVCALFGVSPMVAENLKTLIKPFCMYYHIQCLSCDWGTIVLMLIRFSCAKNRTTIAKCLSTLVNIPQSQMFIEPPKLRSTPVALYFYRTGISNISNTYGETPEWITRQTQLQHSFED | ||||||
Domain | 437-587 | SF3 helicase | ||||
Sequence: VNFMSFIQMFKQFLKGTPKHNCIVIYGPPNTGKSLFAMSLMKFMQGSIISYVNSGSHFWLQPLEDAKIALLDDATYGCWTYIDQYLRNFLDGNPCSIDRKHRSLIQLVCPPLLITSNINPQEDANLMYLHTRVTVLKFLNTFPFDNNGNAV |
Sequence similarities
Belongs to the papillomaviridae E1 protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length634
- Mass (Da)71,685
- Last updated2016-01-20 v1
- ChecksumC429CCBFA515CEE1
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 95-117 | Polar residues | ||||
Sequence: SPLGDITNQNNTHSHSQANESQV |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KF436866 EMBL· GenBank· DDBJ | ALJ32863.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KF436867 EMBL· GenBank· DDBJ | ALJ32870.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KF436868 EMBL· GenBank· DDBJ | ALJ32877.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KF436871 EMBL· GenBank· DDBJ | ALJ32898.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KF436872 EMBL· GenBank· DDBJ | ALJ32905.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KU298902 EMBL· GenBank· DDBJ | ALT54764.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KU298903 EMBL· GenBank· DDBJ | ALT54771.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KU298904 EMBL· GenBank· DDBJ | ALT54778.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KU298905 EMBL· GenBank· DDBJ | ALT54785.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KX514432 EMBL· GenBank· DDBJ | ARQ82732.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861810 EMBL· GenBank· DDBJ | CAD1799156.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861846 EMBL· GenBank· DDBJ | CAD1807082.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861863 EMBL· GenBank· DDBJ | CAD1807199.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861869 EMBL· GenBank· DDBJ | CAD1807244.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861887 EMBL· GenBank· DDBJ | CAD1807377.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861893 EMBL· GenBank· DDBJ | CAD1807420.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861896 EMBL· GenBank· DDBJ | CAD1807442.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861911 EMBL· GenBank· DDBJ | CAD1807549.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861920 EMBL· GenBank· DDBJ | CAD1807613.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861929 EMBL· GenBank· DDBJ | CAD1807676.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861939 EMBL· GenBank· DDBJ | CAD1807742.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR861977 EMBL· GenBank· DDBJ | CAD1813874.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR862023 EMBL· GenBank· DDBJ | CAD1814142.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR862037 EMBL· GenBank· DDBJ | CAD1814248.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR862069 EMBL· GenBank· DDBJ | CAD1814481.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LR862072 EMBL· GenBank· DDBJ | CAD1814509.1 EMBL· GenBank· DDBJ | Genomic DNA |