A0A0N9X7I0 · A0A0N9X7I0_PSEFL

  • Protein
    Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
  • Gene
    glnE
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function[glutamate-ammonia-ligase] adenylyltransferase activity
Molecular Function[glutamine synthetase]-adenylyl-L-tyrosine phosphorylase activity
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Biological Processregulation of glutamine family amino acid metabolic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
  • Alternative names
    • ATP:glutamine synthetase adenylyltransferase
    • ATase

Including 2 domains:

  • Recommended name
    Glutamine synthetase adenylyl-L-tyrosine phosphorylase
  • EC number
  • Alternative names
    • Adenylyl removase
      (AR
      ; AT-N
      )
  • Recommended name
    Glutamine synthetase adenylyl transferase
  • EC number
  • Alternative names
    • Adenylyl transferase
      (AT
      ; AT-C
      )

Gene names

    • Name
      glnE
    • ORF names
      AO356_08955

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • FW300-N2C3
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0A0N9X7I0

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-462Adenylyl removase
Domain50-298Glutamate-ammonia ligase adenylyltransferase repeated
Domain319-457PII-uridylyltransferase/Glutamine-synthetase adenylyltransferase
Region473-983Adenylyl transferase
Domain578-831Glutamate-ammonia ligase adenylyltransferase repeated
Domain853-951PII-uridylyltransferase/Glutamine-synthetase adenylyltransferase

Sequence similarities

Belongs to the GlnE family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    983
  • Mass (Da)
    110,006
  • Last updated
    2016-01-20 v1
  • Checksum
    523A8379195A0363
MSLPSLAPIPEMVPAILQSLVNRAEQSFRAAVASLDDDRGLSAWTPQRWEAFSRIAAASDFVIEQSVRDPAMLLELVRSGELDRSLAPGEMRAQIAAAVQAAETEDELGRVLRRQRTRQQVRIIWRDLNRQADLVQTCRDLSDMADTCIDQAYHWLYQRLSQQFGTPTGRRSGEPQQMVILGMGKLGAVELNLSSDIDLIFAYPEGGETVGVKRPLDNQEFFIRVGQRLIKALDPMTVDGFVFRVDMRLRPYGSAGALVLSFNALEQYYQDQGRDWERYAMIKARVVAGDQVAGAQLLDMLRPFVYRRYLDFSAIEALRTMKQLIQQEVRRKGMADNIKLGSGGIREVEFIAQAFQLIHGGRDLSLQQRPLLKVLSTLEGQGYLPATVIDELRQGYEFLRYTEHAIQAIADRQTQMLPDSPQDQARIAFMLGFADWSAFHEQLMYWRGRVAWHFGQVIADPDEEEGGESEVVVGGEWLPLWEDSQDEGAACRQLEEGGFSDAPKALKTLAALRASPQLRAMQRLGRERLDAFIPRLLAQAVEHADPDLVLERVLPLVEAVARRSAYLVLLTENPGALRRLLTLCAASPWIAEQITRFPLLLDELLNEGRLFKPPLAPELAAELRERLTRIPEDDLEQQMEALRHFKLAHRLRVAASEIAGSLPLMKVSDYLTWLAEAILEQVLALAWRQTAAKHGVPLRTDGSLCDPGFIIVGYGKVGGLELGHGSDLDLVFIHDGDPQAETDGPKPIDGAQFFTRLGQRIIHLLTAQTNSGQLYEVDMRLRPSGASGLLVSSLGAFARYQENEAWTWEHQALVRARVLVGSQDVGQAFEKVRVQILGRKRDLPTLRQEVSEMRAKMRDNLGSKATAAGTAANAFEATAPFDLKQDAGGIVDIEFMVQYAALAWSQEHPSLVRYTDNIRILDGLQELGLMPAEDATLLREAYKAYRSAAHRQALQKDAGVIAGDQFVDERRQVLRIWRELGLS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP012831
EMBL· GenBank· DDBJ
ALI06929.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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