A0A0N9SPY4 · A0A0N9SPY4_HHV8
- ProteinRibonucleoside-diphosphate reductase large subunit
- GeneORF61
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids792 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Catalytic activity
- [thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate
RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:73316 + CHEBI:15377 = RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3+ CHEBI:57930
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 200 | substrate | ||||
Sequence: T | ||||||
Binding site | 215-216 | substrate | ||||
Sequence: SC | ||||||
Site | 216 | Important for hydrogen atom transfer | ||||
Sequence: C | ||||||
Binding site | 246 | substrate | ||||
Sequence: G | ||||||
Active site | 415 | Proton acceptor | ||||
Sequence: N | ||||||
Binding site | 415-419 | substrate | ||||
Sequence: NLCAE | ||||||
Active site | 417 | Cysteine radical intermediate | ||||
Sequence: C | ||||||
Active site | 419 | Proton acceptor | ||||
Sequence: E | ||||||
Site | 431 | Important for hydrogen atom transfer | ||||
Sequence: C | ||||||
Binding site | 606-610 | substrate | ||||
Sequence: PTAGT | ||||||
Site | 737 | Important for electron transfer | ||||
Sequence: Y | ||||||
Site | 738 | Important for electron transfer | ||||
Sequence: Y | ||||||
Site | 782 | Interacts with thioredoxin/glutaredoxin | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | ribonucleoside-diphosphate reductase complex | |
Molecular Function | ATP binding | |
Molecular Function | ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor | |
Biological Process | deoxyribonucleotide biosynthetic process | |
Biological Process | DNA replication | |
Biological Process | viral process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonucleoside-diphosphate reductase large subunit
- EC number
- Short namesR1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Gammaherpesvirinae > Rhadinovirus > Rhadinovirus humangamma8
- Virus hosts
Accessions
- Primary accessionA0A0N9SPY4
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Keywords
- PTM
Expression
Keywords
- Developmental stage
Interaction
Subunit
Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 584-606 | Ribonucleotide reductase large subunit | ||||
Sequence: WDALGKSIAEHGIFNSQFLAVMP | ||||||
Region | 758-781 | Disordered | ||||
Sequence: SPPHSGMKQDGAWLPGPKNPEEQS |
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase large chain family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length792
- Mass (Da)88,864
- Last updated2016-01-20 v1
- Checksum2350FF1D0B9AD572