A0A0N9HTA1 · DOMT1_SINHE

Function

function

O-methyltransferase involved in the biosynthesis of etoposide, a chemotherapeutic compound of the topoisomerase inhibitor family (PubMed:26359402).
Catalyzes the methylation of --5'-demethylyatein to produce --yatein (PubMed:26359402).

Catalytic activity

Biotechnology

Combinatorially expression of Sinopodophyllum hexandrum (mayapple) genes of the podophyllotoxin pathway (e.g. DIR, PLR, SDH, CYP719A23, OMT3, CYP71CU1, OMT1, 2-ODD, CYP71BE54 and CYP82D61) in Nicotiana benthamiana (tobacco) results in the production of the chemotherapeutic compound etoposide.

Pathway

Aromatic compound metabolism; phenylpropanoid biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site193S-adenosyl-L-homocysteine (UniProtKB | ChEBI)
Binding site216S-adenosyl-L-homocysteine (UniProtKB | ChEBI)
Binding site236S-adenosyl-L-homocysteine (UniProtKB | ChEBI)
Binding site237S-adenosyl-L-homocysteine (UniProtKB | ChEBI)
Binding site249S-adenosyl-L-homocysteine (UniProtKB | ChEBI)
Binding site250S-adenosyl-L-homocysteine (UniProtKB | ChEBI)
Active site254Proton acceptor
Active site282
Active site314

GO annotations

AspectTerm
Molecular FunctionO-methyltransferase activity
Molecular Functionprotein dimerization activity
Molecular FunctionS-adenosylmethionine-dependent methyltransferase activity
Biological Processmethylation
Biological Processphenylpropanoid biosynthetic process
Biological Processresponse to wounding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Desmethyl-yatein O-methyltransferase
  • EC number

Gene names

    • Name
      OMT1
    • Synonyms
      Phex13114

Organism names

Accessions

  • Primary accession
    A0A0N9HTA1

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004519051-349Desmethyl-yatein O-methyltransferase

Expression

Tissue specificity

Mostly expressed in stems, and, to a lower extent, in leaves.

Induction

Transiently induced after wounding.

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    349
  • Mass (Da)
    38,175
  • Last updated
    2016-01-20 v1
  • Checksum
    EF3BB96625D6E7D8
MDTRADAEIKAMELIGIGVLPLAMKAIIELNVLEILSKAGPDTQLTAAQIVTDIPTTNPNAGFQLDRILRLLASHSVLSSSITKSGERVYGLTPMCKYFLPDQDGVSLAPMVVTIHDKVLLQSWHYLKDSVLKQGSLPFTEAFGMSPFEYSVSDTRFNKVFNAGMFDHSTLCMRDVLQRYKGFQGLGELVDVGGGTGGSLKMILSQYPNLKGINFDLPHVVADAPSFPGVKHIGGDMFESVPSGDAIFMKWILHDWDDGRCLTLLKNCWNALPEHGKVIIVEWILPSDAATDPTSRRVFTADLMMLAFSEGGKERTLGDYGALAKEAGFTTVKDFPCANGISVIEFHKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KT390155
EMBL· GenBank· DDBJ
ALG05117.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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