A0A0N9HTA1 · DOMT1_SINHE
- ProteinDesmethyl-yatein O-methyltransferase
- GeneOMT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids349 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
O-methyltransferase involved in the biosynthesis of etoposide, a chemotherapeutic compound of the topoisomerase inhibitor family (PubMed:26359402).
Catalyzes the methylation of --5'-demethylyatein to produce --yatein (PubMed:26359402).
Catalyzes the methylation of --5'-demethylyatein to produce --yatein (PubMed:26359402).
Catalytic activity
- --5'-demethylyatein + S-adenosyl-L-methionine = --yatein + H+ + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
Biotechnology
Combinatorially expression of Sinopodophyllum hexandrum (mayapple) genes of the podophyllotoxin pathway (e.g. DIR, PLR, SDH, CYP719A23, OMT3, CYP71CU1, OMT1, 2-ODD, CYP71BE54 and CYP82D61) in Nicotiana benthamiana (tobacco) results in the production of the chemotherapeutic compound etoposide.
Pathway
Aromatic compound metabolism; phenylpropanoid biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 193 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 216 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 236 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 237 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 249 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 250 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 254 | Proton acceptor | ||||
Sequence: H | ||||||
Active site | 282 | |||||
Sequence: E | ||||||
Active site | 314 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | O-methyltransferase activity | |
Molecular Function | protein dimerization activity | |
Molecular Function | S-adenosylmethionine-dependent methyltransferase activity | |
Biological Process | methylation | |
Biological Process | phenylpropanoid biosynthetic process | |
Biological Process | response to wounding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDesmethyl-yatein O-methyltransferase
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Ranunculales > Berberidaceae > Podophylloideae > Podophylleae > Sinopodophyllum
Accessions
- Primary accessionA0A0N9HTA1
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000451905 | 1-349 | Desmethyl-yatein O-methyltransferase | |||
Sequence: MDTRADAEIKAMELIGIGVLPLAMKAIIELNVLEILSKAGPDTQLTAAQIVTDIPTTNPNAGFQLDRILRLLASHSVLSSSITKSGERVYGLTPMCKYFLPDQDGVSLAPMVVTIHDKVLLQSWHYLKDSVLKQGSLPFTEAFGMSPFEYSVSDTRFNKVFNAGMFDHSTLCMRDVLQRYKGFQGLGELVDVGGGTGGSLKMILSQYPNLKGINFDLPHVVADAPSFPGVKHIGGDMFESVPSGDAIFMKWILHDWDDGRCLTLLKNCWNALPEHGKVIIVEWILPSDAATDPTSRRVFTADLMMLAFSEGGKERTLGDYGALAKEAGFTTVKDFPCANGISVIEFHKK |
Expression
Tissue specificity
Mostly expressed in stems, and, to a lower extent, in leaves.
Induction
Transiently induced after wounding.
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length349
- Mass (Da)38,175
- Last updated2016-01-20 v1
- ChecksumEF3BB96625D6E7D8
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KT390155 EMBL· GenBank· DDBJ | ALG05117.1 EMBL· GenBank· DDBJ | mRNA |