A0A0N9HT29 · C71BE_SINHE
- ProteinDesmethyl-deoxy-podophyllotoxin synthase
- GeneCYP71BE54
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids507 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Cytochrome P450 involved in the biosynthesis of etoposide, a chemotherapeutic compound of the topoisomerase inhibitor family (PubMed:26359402).
Catalyzes the conversion of deoxypodophyllotoxin to desmethyl-deoxypodophyllotoxin (PubMed:26359402).
Catalyzes the conversion of deoxypodophyllotoxin to desmethyl-deoxypodophyllotoxin (PubMed:26359402).
Catalytic activity
- --deoxypodophyllotoxin + O2 + reduced [NADPH--hemoprotein reductase] = --4'-desmethyl-deoxypodophyllotoxin + formaldehyde + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Biotechnology
Combinatorially expression of Sinopodophyllum hexandrum (mayapple) genes of the podophyllotoxin pathway (e.g. DIR, PLR, SDH, CYP719A23, OMT3, CYP71CU1, OMT1, 2-ODD, CYP71BE54 and CYP82D61) in Nicotiana benthamiana (tobacco) results in the production of the chemotherapeutic compound etoposide.
Pathway
Aromatic compound metabolism; phenylpropanoid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | phenylpropanoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDesmethyl-deoxy-podophyllotoxin synthase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Ranunculales > Berberidaceae > Podophylloideae > Podophylleae > Sinopodophyllum
Accessions
- Primary accessionA0A0N9HT29
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 1-21 | Helical | ||||
Sequence: MEFLSFPLSSALLIILLFMLV |
Keywords
- Cellular component
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_5006035457 | 1-507 | Desmethyl-deoxy-podophyllotoxin synthase | |||
Sequence: MEFLSFPLSSALLIILLFMLVKKAIQNKNSKLPPGPRKLPVIGSLHHLIGDGLPHHALRNLAKKHGPLMHLQLGENSTLVVSSSKMARAIMSTHDLMFANRTVQFATDILLYGGKGIGLAPYGDYWRQIRKICVLELLSAKRVQSFQTVREEEISNLIRSISAESSKVNFSEMITSLTNDIIARAAFGEKCKDKHAFLSLIEEGIQLAGGFDIAGLFPSLKLLHVITGKKRQLERVHNELDRILVDIIKENKEKRRASKLNEDKYEENLVDVLVRVQENTQLEVPLANDNLKAVILDIFVAGSETSSTTIEWAMSEMLKNPKVMKKAQAEVRRVFSGNKKINETEIQELSYLKLVLKETLRLHAPAPLLIPRECRESCEIDGYEIPKKTMVMVNAWAIGRDPENWSNGDRFEPERFDGISVDYKGTNFEYIPFGAGRRICPGMLFGMANVEVPLARLLYHFDWELPNGIKPEELDMDETFGTTVRRKNHLYLIPTVVHELERSTTKE |
Expression
Tissue specificity
Rhizome-specific expression.
Structure
Sequence
- Sequence statusComplete
- Length507
- Mass (Da)57,408
- Last updated2016-01-20 v1
- Checksum1B37791A87766AAA