A0A0N9HT29 · C71BE_SINHE

Function

function

Cytochrome P450 involved in the biosynthesis of etoposide, a chemotherapeutic compound of the topoisomerase inhibitor family (PubMed:26359402).
Catalyzes the conversion of deoxypodophyllotoxin to desmethyl-deoxypodophyllotoxin (PubMed:26359402).

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Biotechnology

Combinatorially expression of Sinopodophyllum hexandrum (mayapple) genes of the podophyllotoxin pathway (e.g. DIR, PLR, SDH, CYP719A23, OMT3, CYP71CU1, OMT1, 2-ODD, CYP71BE54 and CYP82D61) in Nicotiana benthamiana (tobacco) results in the production of the chemotherapeutic compound etoposide.

Pathway

Aromatic compound metabolism; phenylpropanoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site440Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Biological Processphenylpropanoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Desmethyl-deoxy-podophyllotoxin synthase
  • EC number
  • Alternative names
    • Cytochrome P450 family 71 subfamily BE polypeptide 54

Gene names

    • Name
      CYP71BE54

Organism names

Accessions

  • Primary accession
    A0A0N9HT29

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1-21Helical

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_50060354571-507Desmethyl-deoxy-podophyllotoxin synthase

Expression

Tissue specificity

Rhizome-specific expression.

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    507
  • Mass (Da)
    57,408
  • Last updated
    2016-01-20 v1
  • Checksum
    1B37791A87766AAA
MEFLSFPLSSALLIILLFMLVKKAIQNKNSKLPPGPRKLPVIGSLHHLIGDGLPHHALRNLAKKHGPLMHLQLGENSTLVVSSSKMARAIMSTHDLMFANRTVQFATDILLYGGKGIGLAPYGDYWRQIRKICVLELLSAKRVQSFQTVREEEISNLIRSISAESSKVNFSEMITSLTNDIIARAAFGEKCKDKHAFLSLIEEGIQLAGGFDIAGLFPSLKLLHVITGKKRQLERVHNELDRILVDIIKENKEKRRASKLNEDKYEENLVDVLVRVQENTQLEVPLANDNLKAVILDIFVAGSETSSTTIEWAMSEMLKNPKVMKKAQAEVRRVFSGNKKINETEIQELSYLKLVLKETLRLHAPAPLLIPRECRESCEIDGYEIPKKTMVMVNAWAIGRDPENWSNGDRFEPERFDGISVDYKGTNFEYIPFGAGRRICPGMLFGMANVEVPLARLLYHFDWELPNGIKPEELDMDETFGTTVRRKNHLYLIPTVVHELERSTTKE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KT390179
EMBL· GenBank· DDBJ
ALG05141.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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