A0A0N9HL59 · A0A0N9HL59_9PSEU

Function

function

Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site996Mg2+ 1 (UniProtKB | ChEBI)
Binding site1003Mg2+ 2 (UniProtKB | ChEBI)
Binding site1003Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionlysine-tRNA ligase activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleic acid binding
Molecular Functionphosphatidylglycerol lysyltransferase activity
Biological Processlipid metabolic process
Biological Processlysyl-tRNA aminoacylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lysine--tRNA ligase
  • EC number
  • Alternative names
    • Lysyl-tRNA synthetase
      (LysRS
      )

Gene names

    • Name
      lysS
    • ORF names
      AOZ06_07520

Organism names

  • Taxonomic identifier
  • Strain
    • KLBMP1111
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Kibdelosporangium

Accessions

  • Primary accession
    A0A0N9HL59

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane12-31Helical
Transmembrane43-68Helical
Transmembrane75-96Helical
Transmembrane108-132Helical
Transmembrane144-166Helical

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain766-1079Aminoacyl-transfer RNA synthetases class-II family profile

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,084
  • Mass (Da)
    120,062
  • Last updated
    2016-01-20 v1
  • Checksum
    EE313873E39139FF
MIWGVGKHRAAGIVATIVQVGAFLSVFFVLTDVDSEVRWYVGFASWMVGLPVDENLFISVVLLVLGAALRRRKRAALWTLVVFEVGSVLTDLVYLLTLEEDPDLAVDIGEVTTLLVVAVAVELAIILVLLRLRPSFPAQIAPGAVRRSVAILLVGLAAVTVAGWILTGPFPGSLREVWEKLVWAGNHTTGELLQLRPLGIGRGPGWLGTLLDVLGALVAVVAVYRFFRGVRSMRHLAPDEELKVRELLAEYGERDSLGYFATRRDKSVVFAPNGRAAVTYRVLGGVSVASGDPVGDPEAWRQAVDMWLVEAHRYGWASGVLGASEDGAKVYTAAGLKALEIGDEAILDVRQFTLSGPERRSVRQAVNRIERAGYTCTVRRHSEIPEPEMETLRSAAQHWRGAQTERGFSMALGRLGDPADGRCVMVEAYDLAGRLRGLLSFVPWGRRGLSLDLMRRDRDAGNGLNEFMLNAVIEASGKLGAQRISLNFAMFRAVFEGGERIGAGPVLRAWRGILGVFSRFLQLESLYRSNAKYGPDWEPRFLCYSRTRRLPKVGIVAGALEGFLPTLQRRSGLRAEPVDDQFVGRVREIDELAAKAKPRQARRPEQVRVRIAKLERLRAAGYDPYPPGFDRDTRLSAVVERFPALPPDTRTGQHVRIAGRVIASRDLGGLCFVRLRDFGGELQVMLTGPALHDWRSLVDLGDHVGVSGEVVTSRRGELSVLADEWTVTAKCLHPLPDKRKGLSDPETRVRQRYLDLVVNPESAAMLKLRSTVVRALRETLHDKDFLEVETPMLQTVHGGANARPFVTHINAYDMRMYLRIAPELYLKRLCVAGVERVFELNRNFRNEGVDASHNPEFTMLEAYQAYADYSTMRVLTQKLVQRAARAAYGAEIVRRGGEEFDISGDWPVVSVHQAVSGALGEEITPDTPVRELKGLLAKAGIVLDDELGHGHLVQKAYEYLVEGDTVEPTFYTDFPTETSPLTRRHRVEQRVAERWDLVAFGSEIGTAYTELTDPIEQRERLEAQSLRAASGDVEAMELDEDFLRALEHGMPPTGGMGLGVDRLLMMLTGASIRQTVLFPFARPR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP012752
EMBL· GenBank· DDBJ
ALG06794.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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