A0A0M9DL29 · A0A0M9DL29_9BACI

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site552L-isoleucyl-5'-AMP (UniProtKB | ChEBI)
Binding site596ATP (UniProtKB | ChEBI)
Binding site890Zn2+ (UniProtKB | ChEBI)
Binding site893Zn2+ (UniProtKB | ChEBI)
Binding site910Zn2+ (UniProtKB | ChEBI)
Binding site913Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      ADM90_04480

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • DSM 54
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Lysinibacillus

Accessions

  • Primary accession
    A0A0M9DL29

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain28-632Aminoacyl-tRNA synthetase class Ia
Motif58-68'HIGH' region
Motif593-597'KMSKS' region
Domain676-833Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Domain887-916Zinc finger FPG/IleRS-type

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    922
  • Mass (Da)
    104,782
  • Last updated
    2015-12-09 v1
  • Checksum
    BDC687F112CFA221
MVEYKDTLLMPKTDFPMRGNLPANEPKMQEKWNDMDINKLQMERTEGRPEFVLHDGPPYANGDIHIGHALNKVLKDMITRHRSMSGYHVNYVPGWDTHGLPIEQALTNKGVKRKEMSVAEFRQLCEKYAYEQIDNQRQQFKRLGIRGDWENPYITLKPEFEARQIEVFGKMAEKGYIYKGLKPVYWSPSSESALAEAEIEYKDIKSPSIYVSFAIKDAKGVVPVDAKFIIWTTTPWTLPANLGISLNPEFIYVVVAVANKKFIIAKELLESVATTLGWENYEVVQEVKGEALDRLVAQHPFYDRESLIMVGEHVTAEAGTGCVHTAPGHGEDDYHIGKQYGLGILSPVDNSGCYTDEAPGFEGVFYNDANKLITEKLKEVEALEKLDFFTHSYPHDWRTKKPVIYRATPQWFASVEAFRGELLEAVKATTFTPAWGETRLYNMIRDRGDWVISRQRAWGVPIPIFYAENGEPIITPETITHVAALFREHGSNIWFQKEPKELLPEGFTHPGSPNGEFTKENDIMDVWFDSGSSHQGVLVERGMKYPADLYLEGSDQHRGWFNSSLITSVAINGYAPYKGLLTHGFVLDGEGRKMSKSLGNVIIPQKVMDQYGADILRLWVASVDYTADVRISMDMLKQVSEVYRKIRNTLRFLHGNIADFDPKKDRVAYAELREMDQYVYMRLQDVLKTVRAAYERYDFAAVYHVVNNFVAVELSSFYLDIAKDVVYIEGYDNQDRRAMQTVIYDTLMTLVKVMTPIIPHTTDEMWSYLYAQGLVEEASVQLTDFPEVDEQANFDSLREKWEKVIDVRDDILKALEEARNAKIIGKSLEAKVTVYAKDDIVALLNDTTIDFAQLSIVSAFEVAALAEAPDHALALEHVSIVVEKAAGEKCERCWSISETVGANEAQPTVCARCATVVEKYYA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LGCI01000005
EMBL· GenBank· DDBJ
KOY82600.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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